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Skin is a kind of tissue that surrounds the surface of body, it is the first barrier for animals to resist mechanical, chemical and pathogenic microorganisms. Skin wound is one of the most common surgical diseases. The process of wound healing can be summarized as three stages: inflammation stage, fibrous tissue proliferation stage, and scar formation and repair stage. Incomplete repair of the wound leads to skin scarring, which causes the tissue to lose its normal structure and function, and seriously affects the aesthetic appearance. Traditional treatment methods can not restore the normal function of the skin and have obvious adverse reactions, which can not meet people's needs. Stem cell therapy, especially adipose derived stem cells (ADSCs) plays a essential rule in the process of wound healing making it a research hotspot in recent years. ADSCs can secrete a variety of growth factors during wound healing to reduce wound inflammatory response, promote wound regeneration epithelialization and vascular reconstruction, thereby promoting wound healing. In this paper, the wound healing process and its regulation mechanism were summarized, and the role of ADSCs in wound healing at home and abroad and its clinical application progress were reviewed.
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The periosteum is a special connective tissue enveloping bone,which not only contains the mesenchymal cells required for bone repair,i.e.periosteum derived stem cells (PDSCs),but also provides the microenvironment required for PDSCs and the necessary biomechanical support.Periostea play a vital role in bone tissue repair.Clinical periosteal transplantation has been widely applied to restore bone defects,which is an important research field in regenerative medicine.In this paper,the structure characters of periosteal cells isolation and characterization of PDSCs were reviewed.The research progress of periosteum and PDSCs in bone defect restoration was reviewed.The related signal pathways involved in the restoration of PDSCs were discussed.Periostea and PDSCs are not only crucial for bone defect repair but also play important role in bone regeneration.The differences in proliferation and differentiation potential of PDSCs in human/mouse amputation,the amphibian epimorphic regeneration and the annual regeneration of deer antler were compared and analyzed.The results showed that the proliferative potential of PDSCs and abundant blood supply may be the key factors determining bone regeneration.
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Objective To investigate the effect of human papillomavirus (HPV) 16 E6 gene on the expression of E-cadherin (E-cad) in cervical cancer cell line SiHa. Methods HPV16 E6 expression system was established in the cervical cancer cell line SiHa by using transient transfection system, MTT method was used to detect SiHa cell proliferation activity, and reverse transcription-polymerase chain reaction (RT-PCR) and western blot method was used respectively to detect E-cad mRNA and protein expression level in cells after HPV16 E6 transfection. Results Compared with the blank control group (non-transfected plasmid) and the vector group (the addition of pcDNA3.1), the cell viability rate of the E6 group (pcDNA3-1-HPV16 E6) was significantly increased (P<0 .05), while there was no significant difference between the vector group and the blank control group (P>0.05). The relative expressions of E-cad mRNA in the E6 group, the vector group and the blank control group were 0.26±0.12, 0.82±0.14, 0.83±0.21 respectively, then the protein relative expressions in the three groups were 0.62±0.02, 1.33±0.04, 1.31±0.05 respectively. The expressions level of E-cad mRNA and protein in E6 group were significantly lower than those in the other two groups (all P<0.05). However, there was no significant difference between the vector group and the blank control group (both P>0.05). Conclusion The instantaneous transfection of HPV16 E6 gene can reduce the expression of E-cad in cervical cancer cells, which is closely related to the occurrence and development of cervical cancer.
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Objective To investigate the effect of human papillomavirus (HPV) 16 E6 gene on the expression of E-cadherin (E-cad) in cervical cancer cell line SiHa. Methods HPV16 E6 expression system was established in the cervical cancer cell line SiHa by using transient transfection system, MTT method was used to detect SiHa cell proliferation activity, and reverse transcription-polymerase chain reaction (RT-PCR) and western blot method was used respectively to detect E-cad mRNA and protein expression level in cells after HPV16 E6 transfection. Results Compared with the blank control group (non-transfected plasmid) and the vector group (the addition of pcDNA3.1), the cell viability rate of the E6 group (pcDNA3-1-HPV16 E6) was significantly increased (P<0 .05), while there was no significant difference between the vector group and the blank control group (P>0.05). The relative expressions of E-cad mRNA in the E6 group, the vector group and the blank control group were 0.26±0.12, 0.82±0.14, 0.83±0.21 respectively, then the protein relative expressions in the three groups were 0.62±0.02, 1.33±0.04, 1.31±0.05 respectively. The expressions level of E-cad mRNA and protein in E6 group were significantly lower than those in the other two groups (all P<0.05). However, there was no significant difference between the vector group and the blank control group (both P>0.05). Conclusion The instantaneous transfection of HPV16 E6 gene can reduce the expression of E-cad in cervical cancer cells, which is closely related to the occurrence and development of cervical cancer.
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BACKGROUND:Deer antlers are the unique mammalian organs which can periodical y regenerate, and the process is known as a stem cel-based event. Exploring the underlying mechanism of deer antler regeneration and indentifying the functional role of stem cellin mammalian organ regeneration are of great importance to regenerative biology and regenerative medicine. OBJECTIVE:To review the relevant literatures of the research progress in antler regeneration, as wel as effects of stem cells and cytokines on antler regeneration. METHODS:A computer-based online search of PubMed (1994-01/2012-10) was performed for acquiring the articles in English by using the key words of“deer antler;antler regeneration;stem cell. In addition, manual search was also performed for those literatures that cannot be readily obtained from internet search. Articles concerning antler regeneration histology, morphology, antler stem cells and micro-environmental studies, and related cytokines. Repetitive studies or articles that are unrelated to the criteria set for the article were excluded. RESULTS AND CONCLUSION:A total of 87 articles were obtained and final y 31 articles were selected. The key tissue types for antler regeneration are antlerogenic periosteum and pedicle periosteum, the cells within which are known as antler stem cells. The covering skin of antlerogenic periosteum and pedicle periosteum constitutes the functional niche for antler stem cells. Numerous cytokines are involved in the process of antler fast growing and ful regeneration, including insulin-like growth factor, sex hormones, human epidermal growth factor, and vascular endothelial growth factor. It is vital y important to identify the interacting molecules between the antler stem cells and their niche celltypes, and to define the role of each molecule that plays in antler regeneration, which wil greatly advance our knowledge of the stem cel-based mammalian organ regeneration.
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Objective To investigate the role of pulmonary ventilatioa/perfusion imaging in evaluating thrombolytic therapy in pulmonary embolism (PE).Methods Pulmonary ventilation/perfusion imagng was performed before and one week and three months after thrombolytic therapy in 43 patients with acute PE.Results Among 421 abnormal pulmonary segments in 43 PE cases,199(47.3%) and 231(54.9%) segments were restored to normal 1 week and 3 months later (P<0.05).Of two groups,more abnormal pulmonary segments were restored to normal in those with less than one week's onset (P<0.01).Conclusion As a non-invasive diagnostic method, pulmonary ventilation/perfusion imaging plays an important role in evaluating the therapeutic effectiveness of thrombolytic therapy in patients with PE.
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The enzymatic synthesis of endomorphins were carried out by immobilized papain on sodium alginate-chitosan (IPSAC). The reaction has been carried out in two steps. First, Trp-Phe-NH2 was obtained by Boc-Trp-OH coupling with Phe-NH2 using IPSAC catalyst in microaqueous acetonitrile system with a 27.8 % yielding as determined by high performance liquid chromatography (HPLC). The catalytic properties of IPSAC were examined by studying the dependence of pH, ionic strength, solution content, reaction temperature,enzyme loading and reaction time over the yields. The results of orthogonal experiments indicated that pH was the most significant factor that influenced this synthesis. Second, Boc-Tyr-Pro-OMe and Trp-Phe-NH2 were suspended into the microaqueous acetonitrile to produce Tyr-Pro-Trp-Phe-NH2 (endomorphin-1) with a 35.2% yield.
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Deer antlers are the unique mammalian organs that can be periodically regenerated,i.e.,they hold interest for researchers on stem cells and regenerative medicine,and have the potential to become an excellent biomedical research model.Deer antler regeneration may use stem cells.Antler growth appears to involve specific stimulation of the necessary stem cells present in the locality,and involves similar mechanisms to those used in limb development,unlike the regenerative process in the newt.Development regulation of antler and growth mechanism of antler stem cells have significance in limb regenerated medicine and stem cell research.Many scientists have carried out thorough research on the deer antlers development,thus,have drawn many concepts.This article reviews the field of deer antlers development such as antler regeneration and stem cells,and introduces research technique of antler development and relevant theories.
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<p><b>OBJECTIVE</b>To identify and characterize laryngeal cancer related novel genes located on chromosome 6q25.</p><p><b>METHODS</b>Electric hybridization was performed in human genome database using EST (expression sequence tag) as probe. Novel genes were deduced by software from positive DNA clones and their cDNAs were amplified by RT-PCR using primers designed according to the sequence of the putative genes.</p><p><b>RESULTS</b>A novel gene was cloned successfully. The full length of this gene was about 21 kb. It contained two exons and produced a 1006 bp transcript coding a protein with 235 amino acid residues. It's 5'flanking sequence contained two binding sites of oncoprotein c-Myc, thus it was named MTLC (c-Myc target from laryngeal cancer cells). Homologous assay showed that MTLC exhibited little overall homology to known human proteins but it exhibited good overall homology to mouse MT-MC1 protein with an identity of 78%. The primary structure of MTLC protein contained a nuclear location signal motif, but it did not have other conserved domains. The results of subcellular location experiment showed that MTLC expressed in nuclei of human hepatocellular carcinoma cell line Bel7402 cells, while a wide distribution of MTLC in various tissues was demonstrated by Northern blotting.</p><p><b>CONCLUSION</b>MTLC may play an important role as a target gene of c-Myc and as a transcription factor in keeping the normal physiological process of cells.</p>
Subject(s)
Humans , Amino Acid Sequence , Base Sequence , Blotting, Northern , Chromosomes, Human, Pair 6 , Genetics , Cloning, Molecular , DNA, Complementary , Chemistry , Genetics , Gene Expression , Green Fluorescent Proteins , Laryngeal Neoplasms , Genetics , Luminescent Proteins , Genetics , Metabolism , Microscopy, Fluorescence , Molecular Sequence Data , Nuclear Proteins , Genetics , Recombinant Fusion Proteins , Genetics , Metabolism , Sequence Alignment , Sequence Analysis, DNA , Sequence Homology, Amino Acid , Tumor Cells, CulturedABSTRACT
Questionnaire was made to investigate teaching effect and further improve teaching quality of medical genetics experiment.The results showed that the refined experimental contents and reasonable teaching methods were vital to the teaching effect.Furthermore,the ability of independent thinking and operating skills should be considerably emphasized.
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Objective To evaluate the diagnostic value of three nuclide imaging methods in patients with hyperparathyroidism. Methods Thirty five patients with hyperparathyroidism underwent 201 Tl/ 99m TcO-4 (8 cases), double phase 99m Tc MIBI imaging methods (27 cases) and 99m Tc MIBI/ 99m TcO-4 substraction imaging (20 cases). Abnormal increase of radioactivity in substraction imaging or delay imaging denoted positive result. All data of nuclide imaging were evaluated according to final clinical results and were compared with ultrasound or CT. Results 35 cases of hyperparathyroidism were proved, including 31 adenomas (ectopic 1), 3 hyperplasia and 1 carcinoma. The sensitivity of 201 Tl/ 99m TcO-4 , double phase 99m Tc MIBI imaging and 99m Tc MIBI/ 99m TcO-4 substraction imaging was 62.5%, 88.9%,90.0%, respectively. The specificity of 3 nuclide imaging methods was 100%. The sensitivity and specificity of ultrasound were 74.3%, 85.7%, respectively. The sensitivity of CT was 78.6%. The results showed that 99m Tc MIBI/ 99m TcO-4 was superior to other imaging. Conclusion An accurate diagnosis of hyperparathyroidism and preoperative anatomic localization can be determined by means of nuclide imaging.