ABSTRACT
In the development of oxidative stress-relevant diseases, reactive oxygen species (ROS) removal obstacle or excess production results in the damage of the body tissues and organs. Recent studies have demonstrated that nuclear factor E2-related factor 2/heme oxygenase-1 (Nrf2/HO-1) axis played a significant role in anti-oxidative stress. The Nrf2/HO-1 axis counteracts oxidative stress injury by its resistance to inflammation, oxidation, mitochondrial damage and calcium influx, apoptosis, pyroptosis, ferroptosis and autophagy, which provides a theoretical basis for its therapeutic effect on various oxidative stress-relevant diseases in multiple organs (respiratory, cardiovascular, nervous, digestive, urinary and blood systems). Therefore, effective regulation of the Nrf2/HO-1 signal axis can be an important strategy for treatment of oxidative stress-relevant diseases.
Subject(s)
Heme Oxygenase-1 , NF-E2-Related Factor 2 , Oxidative Stress , Reactive Oxygen Species , Signal TransductionABSTRACT
OBJECTIVE@#To explore the changes of mTOR signal pathway in HeLa cells under different nutritional conditions infected with Coxsackie virus B3 (CVB3).@*METHODS@#The HeLa cells were cultured with two methods: the conventional culture method cultured HeLa cells with medium with 10% fetal bovine serum for 24 h and changed the medium next day, and then infected with CVB3; the serum starvation method cultured HeLa cells with medium without fetal bovine serum for 24 h, and then infected with CVB3. The expression of the coat protein of CVB3, mTOR, p70S6K mRNA was detected with RT-PCR at different time points.@*RESULTS@#The virus group showed the expressions of mTOR and p70S6K mRNA were significantly higher than those in the control group at 12 h and 24 h (P0.05).@*CONCLUSION@#CVB3 can down-regulate the expressions of mTOR and p70S6K mRNA. The mTOR expression in the starvation serum is higher than that in the conventional culture.
Subject(s)
Humans , Cell Culture Techniques , Down-Regulation , Enterovirus B, Human , Virulence , HeLa Cells , RNA, Messenger , Genetics , Metabolism , Signal Transduction , Physiology , TOR Serine-Threonine Kinases , Genetics , MetabolismABSTRACT
OBJECTIVE@#To study the mechanism of osteopontin (OPN) in viral myocarditis by observing the expression of OPN and collagen I (Col I) in mice myocardium.@*METHODS@#The viral myocarditis models were achieved by infection with myocarditic coxsackievirus B3 (CVB3). The myocardium of mice was stained by HE and Masson staining, and the pathological scores and the collagen volume fraction (CVF )of myocardium were tabulated. The expression of Col I mRNA was measured by RT-PCR. The expression of OPN was detected by RT-PCR and ELISA.@*RESULTS@#The histopathological examination revealed a prevalence of myocardial cell necrosis and obvious inflammation changes at the 7th day post-infection. Subsequently the inflammatory lesions were gradually absorbed. At the 28th day, the inflammatory cells had almost disappeared and obvious fibrosis occurred. The pathological scores and the expression of OPN mRNA were higher than those of the control group (P<0.05), and reached the highest level at the 7th day (P<0.05). From the 14th day, these parameters decreased,reflected also in the ELISA results. At the 7th day and the 14th day, the Col I expression was similar to that of control. Col I expression at the 21th and 28th days was higher than those of the control (P<0.05), and correlated positively to the CVF results.@*CONCLUSION@#The OPN mRNA expression increased in acute stage of VMC, and higher than that of the control group when in recovery stage, suggesting that OPN might be related to the inflammatory response in acute stage of, and promote the collagen synthesis of recovery stage.
Subject(s)
Animals , Male , Mice , Collagen Type I , Genetics , Metabolism , Coxsackievirus Infections , Metabolism , Enterovirus B, Human , Mice, Inbred BALB C , Myocarditis , Metabolism , Virology , Osteopontin , Genetics , Metabolism , RNA, Messenger , Genetics , MetabolismABSTRACT
Objective To study the effects of mammalian target of rapamycin(mTOR)transfection on the proliferation of NIH3T3 fibroblasts.Methods The plasmid of pcDNA3-mTOR was transfected into NIH3T3 fibroblasts with electroporation method.The positive cell clones were selected with G418.The stable mRNA and protein expressions of mTOR in the cells were determined by RT-PCR and Western blotting analysis respectively.MTT assay was employed to observe the proliferation of NIH3T3 fibroblasts.Results mTOR mRNA and protein expressions increased obviously in transfected group than that of in control group(P