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1.
Mem. Inst. Oswaldo Cruz ; 88(1): 111-7, jan.-mar. 1993. tab, ilus
Article in English | LILACS | ID: lil-117658

ABSTRACT

Non-viable cells and biochemical fractions from Paracoccidioides brasiliens were obtained for experimental inoculation in mice and posterior histopatological analysis. Dead total fungus, total fungus disrupted by sonorous waves, lipids of the fungus, supernatant of the lipid purification, integral and disrupted fungus free of lipids were obtained. The six preparations arised from masses of lyophilized yeasts of a recent isolate of P. brasiliensis (strain JT-1) and from a "Pool" equitably constituted by four strains maintained in laboratory for a long time (SN, 2, 18 and 192). Different doses of the 12 preparations were intraperitonially inoculated and histopathological analysis were done 30 days later. This analysis showed that all the inoculated preparations gave origin to inflamatory foci, except the one designated "supernatant of lipid purification". The alterations were detected exclusively in the liver of the animals and occurred from the smallest dose tested (1 mg), with exception of the lipids of the fungus, where the foci appeared only from a 3 mg dose onwards. No difference in the capacity of inducing histopathological alterations was found between the preparations obtained from the recent isolate (JT-1) and from the older ones ("Pool"). On the other hand, an increase of the number of inflammatory foci in function of the inoculated dose was observed


Subject(s)
Rats , Animals , Paracoccidioides/isolation & purification
2.
Rev. Soc. Bras. Med. Trop ; 25(4): 251-5, out.-dez. 1992. tab
Article in Portuguese | LILACS | ID: lil-141221

ABSTRACT

Realizou-se um levantamento soroepidemiológico na cidade de Londrina - Paraná, para leptospirose, em 49 indivíduos näo expostos a risco, 75 trabalhadores da limpeza pública, 55 indivíduos com atividade em ambiente hospitalar e 29 trabalhadores do departamento de Agua e Esgoto. Dos 208 soros analisados pela reaçäo de soroaglutinaçäo microscópica, 28,4 por cento apresentaram aglutininas antileptospira. A maior positividade foi encontrada nos soros dos trabalhadores da limpeza pública (46,7 por cento)


Subject(s)
Humans , Agglutinins/blood , Leptospira/immunology , Occupations , Urban Population , Agglutination Tests , Brazil/epidemiology , Leptospirosis/epidemiology , Leptospirosis/immunology , Occupations/statistics & numerical data , Urban Population/statistics & numerical data , Seroepidemiologic Studies
3.
Rev. microbiol ; 14(3): 215-22, 1983.
Article in Portuguese | LILACS | ID: lil-17672

ABSTRACT

Em 11 setores do Hospital das Clinicas da Universidade Federal de Minas Gerais, previamente selecionados quanto as condicoes de contaminacao e controle de acesso ao publico, subdivididos em 49 ambientes de coleta, foram analisados material do ar e do piso, nos horarios da manha e da tarde, com auxilio de placas de Petri com Agar-Sabouraud-Glicose e cotonetes esterilizados para a colheita do material do piso no periodo de 17 de abril a 29 de maio 1980, intercalados de 3 em 3 dias, perfazendo um total de 2940 colheitas. Dentre 9064 colonias isoladas foram identificados 48 generos; 26,90% dos fungos nao apresentaram orgao de frutificacao e foram classificados como Cladosporium (65,03%) Aspergillus (37,08%), Fusarium (22,10%), Penicillium, (19,86%), Aureobasidium (18,40%), Curvularia (16,20%), Nigrospora (15,30%). No ar e no horario da manha, foi observada uma maior quantidade de fungos. Os ambientes considerados contaminados, apresentaram maior concentracao dos mesmos. Entretanto, na cirurgica, onde se deveria esperar uma menor quantidade de esporos fungicos ocorreu o inverso


Subject(s)
Air Microbiology , Fungi , Hospitals, General , Brazil
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