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Objective To investigate the effect of aloperine on the proliferation and apoptosis of bladder cancer EJ cells and to explore its mechanism.Methods;The experimental group was as follows:bladder cancer EJ cells were not treated (group A);25μmol/L aloperine was added (group B);50μmol/L aloperine was added (group C).100μmol/L aloperine was added (group D).The survival rate of the cells was detected by CCK8.The apoptosis rate was detected by flow cytometry.The expression levels of Bcl-2,Bax,p Erk1/2 protein were detected by Western blot.Results CCK8 results showed that:B,C,D group compared with A group,the cell survival rate were decreased (P ＜ 0.05).The results of flow cytometry showed that the apoptosis rate of B,C and D group was higher than that of A group (P ＜ 0.05).Western blot results showed that:B,C,D group compared with A group the expression of Bcl-2 and p Erk1/2 levels were reduced (P ＜ 0.05),the expression of Bax level was increased (P ＜ 0.05).Conclusion The survival rate of EJ in bladder cancer can be reduced by aloperine and induce its apoptosis,and inhibiting the phosphorylation of Erk1/2 may be the mechanism.
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Objective To observe the effects of Yishen Tongluo Decoction (YTD) on the renal mRNA expression of transforming growth factor-β1 ( TGF-β1) and collagen Ⅳ ( ColⅣ) in membranous nephropathy ( MN) rats. Methods SD rats were randomly divided into normal group, model group, benazepril group (in the dosage of 10 mg·kg-1·d-1) , YTD group ( in the dosage of 20 g·kg-1·d-1) . The rats in various groups were given intragastric administration of corresponding agents. At the end of the fourth week, 24-hour urinary protein quantity, albumin ( ALB) , total protein ( TP) , triglyceride ( TG) , total cholesterol ( TC) , blood urea nitrogen ( BUN) , and creatinine (Cr) levels were observed. The mRNA expression levels of TGF-β1 and ColⅣ in renal tissue of rats were detected by immunofluorescence method, electron microscopy and real-time polymerase chain reaction (PCR) method. Results In the model group, urinary protein quantity in rats was increased, serum levels of TP and ALB were significantly lowered, serum levels of TC and TG were significantly increased, renal pathological changes were present, and mRNA expression levels of TGF-β1 and ColIV in renal tissue were up-regulated (P0.05) . Conclusion The therapeutic mechanism of YTD for MN is probably related with the inhibition of mRNA expression of TGF-β1 and ColⅣin renal tissue.
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Aurora kinases are a family of serine/threoning kinases whose structures and functions are highly conserved in different model organisms. They play significant roles in many events during cell mitosis, such as centrosome maturation and separation, spindle assembly and maintenance, chromosome segregation, cytokinesis. Overexpression of aurora kinases has been observed in some tumor ceils and aberrations in aurora kinases have been proved to be strongly associated with tumorigenesis. Up to now, some small molecule aurora kinase inhibitors with anti-tumor activity have been developed. Some of those with promising pre-clinical results have reached clinical trial. This review describes the recent progress of aurora kinases and their small molecule inhibitors.
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<p><b>OBJECTIVE</b>To investigate clinical efficacy and feasibility of double-plating fixation via anteriormiddle approach in treating type C3 distal femoral fractures.</p><p><b>METHODS</b>From August 2008 to August 2011, 12 cases with type C3 distal femoral fractures were treated, including 5 open fractures and 7 closed fractures. Among them, there were 8 males, 4 females with an average of 40 years (ranged, 25 to 55 years). There were 7 in left side, 5 in right side. Nine cases were caused by car accident, 3 cases by falling down. The duration from injury to hospital was form 20 minutes to 5 days (mean 135 min). After tibia bone traction for 5 to 8 days, the operation were performed by double-plating fixation via anteriormiddle approach, and autograft of iliac bone or allograft bone grafting were given to bone defect. Knee joint function was evaluated according to Merchanetal criteria.</p><p><b>RESULTS</b>The operation time was from 110 to 160 min, with an average of 135 min, the blood loss was from 300 ml to 500 ml,with an average of 400 ml. Post-operative wound were stage I healing. All patients were followed up from 16 to 36 months (mean 24 months). No infection, reduction loss, nonunion, deep vein thrombosis occurred. Bone healing time was for 18 to 24 weeks with an average of 21 weeks. According to the Merchanetal criteria, 4 cases got excellent results, 6 good, 1 fair and 1 poor.</p><p><b>CONCLUSION</b>Double-plating fixation via anteriormiddle approach for type C3 distal femoral fractures is an effective way, which has advantages of obvious exposure, simple manipulation, anatomical reduction, stable fixation. However,operation indications and operating instructions should be strictly followed.</p>