ABSTRACT
Ten constituents have been isolated from the rhizomes of Cimicifuga foetida L.. Based on spectral evidence and by direct comparison with authentic samples, they were identified as isoferulic acid(Ⅰ ), 3-acetylcaffeic acid(Ⅱ ), caffeic ester glucoside(Ⅲ), cimifugin(Ⅳ),cimifugin glucoside(Ⅴ ), 6 -isoinosine (Ⅵ), cimidahurine(Ⅶ ), cimidahurinine(Ⅷ), D-glucose (Ⅸ) and sucrose (Ⅹ ).
ABSTRACT
For experimental research cn malaria, it is necessary to have a fairly high and relatively stable infection rate of the mosquite vectors with malaria parasite. Many factors are known to exert influence on the sporogeny of P.yoelii, so far, we have made observations only on(1) the influence of splenectomy on the course of infection of mice (Kuming strain) with P.ycelii; (2) that of P-amincbenzoic acid on its sporogonic stages; and(3) the rhyrnic periodicity of infectivity of the gametocytes of the parasite to the mosquitoes The results obtained are reported as follows:(1)It was found that in the splenectomized animals, their parasttemia reached a peak level 6 days after inoculation (3?107-4.5?107 parasitized cells inoculated) and remained at a high level through to the end. The animals began to die from day 8 onwards and finally a total of 7 out of 10 test animals died of the infection within 20 days. However, in the intact animals infected with the same parasites the parasitemia gradually peaked in 8-12 days after inoculation and then dropped rapidly. All of them became latent by day 22 without death at all(2)Two to four days after blood inoculation intact mice were found most infective to the anophline mosquitoes with a gland infection rate of about 40%. The mosquitoes feeding on the splenectomized infected mice during the same period gave rise to, however, a higher gland infection rate than the former. The later the time want on, the more statistically significat the difference in the rate between the two groups of mosquitoes became(P