ABSTRACT
Objective: To investigate the effect of berberine on angiogenesis and signal transduction pathway of hypoxia-inducible growth factor-1alpha [HIF-1 alpha] / vascular endothelial growth factor [VEGF] in rats with cerebral ischemia-reperfusion injury. Study Design: An experimental study. Place and Duration of Study: Luoyang Central Hospital Affiliated with Zhengzhou University, China, from 2016 to 2017
Methodology: Forty-five rats were randomly divided into control group, model group and berberine group, 15 rats in each group. The model of rat with cerebral ischemia-reperfusion injury was duplicated by suture method. Neurological score was evaluated before, after operation, and 7 days after administration. Microvessel density [MVD] of cerebral ischemiareperfusion cortex was detected by immunohistochemistry. HIF-1 alpha mRNA and VEGF mRNA of cerebral ischemia reperfusion cortex were detected by RT-PCR. HIF-1 alpha, and VEGF protein expression of cerebral ischemia-reperfusion cortex was detected by Western Blotting
Results: After operation and 7 days of administration, the neurological scores of the control group, model group, and berberine group were different [all p<0.001]. After 7 days of administration, neurological score of berberine group was lower than that of model group [p<0.001], MVD, HIF-1 alpha mRNA and VEGF mRNA expression. HIF-1 alpha and VEGF protein expression levels were lower in model group than berberine group [all p<0.001]
Conclusion: Berberine can promote angiogenesis in the rat with cerebral ischemia-reperfusion injury. Its mechanism may be activation of HIF-1 alpha / VEGF signal transduction pathway
ABSTRACT
Objective: To investigate the effect of berberine on angiogenesis and signal transduction pathway of hypoxia-inducible growth factor-1Alpha [HIF-1Alpha] / vascular endothelial growth factor [VEGF] in rats with cerebral ischemia-reperfusion injury
Study Design: An experimental study
Place and Duration of Study: Luoyang Central Hospital Affiliated with Zhengzhou University, China, from 2016 to 2017
Methodology: Forty-five rats were randomly divided into control group, model group and berberine group, 15 rats in each group. The model of rat with cerebral ischemia-reperfusion injury was duplicated by suture method. Neurological score was evaluated before, after operation, and 7 days after administration. Microvessel density [MVD] of cerebral ischemiareperfusion cortex was detected by immunohistochemistry. HIF-1Alpha mRNA and VEGF mRNA of cerebral ischemiareperfusion cortex were detected by RT-PCR. HIF-1Ñ, and VEGF protein expression of cerebral ischemia-reperfusion cortex was detected by Western Blotting
Results: After operation and 7 days of administration, the neurological scores of the control group, model group, and berberine group were different [all p<0.001]. After 7 days of administration, neurological score of berberine group was lower than that of model group [p <0.001], MVD, HIF-1Alpha mRNA and VEGF mRNA expression. HIF-1Alpha and VEGF protein expression levels were lower in model group than berberine group [all p <0.001]
Conclusion: Berberine can promote angiogenesis in the rat with cerebral ischemia-reperfusion injury. Its mechanism may be activation of HIF-1Alpha / VEGF signal transduction pathway
ABSTRACT
BACKGROUND:There is a close relationship between epilepsy and apoptosis. The appearance of epilepsy can lead to the loss of neurons in the hippocampus, triggering a series of programmed cel death. OBJECTIVE:To investigate the effect of bone marrow stromal stem cel transplantation on apoptosis in epilepsy. METHODS:After modeled to be of epilepsy 45, Sprague-Dawley model rats were randomly divided into three groups, fol owed by given no intervention (moldel group), normal saline (normal saline group) or bone marrow stromal stem cel transplantation (transplantation group). At 1, 2 and 4 weeks after modeling, the number of Bax-positive cel s, Bcl-2-positive cel s and Bax/Bcl-2 were detected by immunohistochemistry. RESULTS AND CONCLUSION:The number of Bax-positive cel s, Bcl-2-positive cel s and Bax/Bcl-2 presented no obvious changes in the normal saline group at different time points. However, the number of Bax-positive cel s and Bax/Bcl-2 in the transplantation group was significantly decreased, while the number of Bcl-2-positive cel s significantly increased compared with the other two groups at 1, 2 and 4 weeks after modeling (P<0.05). Moreover, the above indicators varied significantly in the transplantation group at different time points after modeling (P<0.05). These results show that bone marrow stromal stem cel transplantation can affect the apoptosis and effectively reduce the apoptosis in rats with epilepsy by up-regulating the number of Bax-positive cel s and down-regulating the number of Bcl-2-positive cel s.
ABSTRACT
Objective To observe the effect of Fasudil on inflammatory factors (TNF-α and IL-8) secreted by astro-cytes.Methods Primary rat spinal astrocytes were cultured and randomly divided into three groups :control group ,oxygen-glu-cose deprivation group (OGD group) and Fasudil group.The culture media were harvested at different time points (0 ,3 ,6 ,12 , 24 h) for detection of the concentration of TNF-αand IL-8 by ELISA.Results Compared with the control group ,the concentra-tions of TNF-αand IL-8 in OGD group were significantly increased at each time point (P<0.05);in Fasudil group ,the concen-trations of TNF-αand IL-8 increased at some time points ,but they were much lower than in OGD group (P<0.05) .Conclusion OGD can promote the secretion of TNF-αand IL-8 by astrocytes in vitro ,which can be effectively inhibited by Fasudil.It is suggested that Fasudil plays an important role in regulating the levels of TNF-αand IL-8 secreted by astrocytes.
ABSTRACT
@#ObjectiveTo study the the simplified rehabilitation procedures characternized with early ambulation for the acute stroke hemiplegic patients without disturbance of consciousness or cognition. Methods40 patients were divided into 2 groups: observed group (20 cases) and control group (20 cases). The patients of observed group were trained with the simplified rehabilitation procedures, while the patients of control group were trained with routine rehabilitation training program. ResultsThe time and costs of rehabilititation training decreased in the observed group compared with those of control group （P＜0.05）. ConclusionThe simplified rehabilitation procedures may benefite the acute stroke hemiplegic patients without disturbance of consciousness and cognition.