ABSTRACT
Objective To establish a method for the determination of perchlorate in food by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).Methods The perchlorate residue in spices and condiments was extracted with water,that in vegetables and fruits was extracted with acetonitrile-water (1∶ 1,V/V),and that in meat,poultry,eggs,milk and aquatic products was extracted with acetonitrile-water (2∶ 1,V/V).The supernatant was cleaned up with C18 SPE (3 ml,200 mg),and the detection was carried out by UPLC-MS/MS with internal standardmethod for quantification.Results The calibration curve was linear in the concentration range of 0.3-20.0 pg/L (R2 ≥0.999),the recovery was in the range of 82.6％-108.6％,the relative standard deviation (RSD) was in the range of 1.0％-9.9％,and the limit of detection was 2.0 μg/kg for milk,and 10.0 μg/kg for other food.Conclusion The method was simple,accurate and highly sensitive,and suitable for the determination of perchlorate in food.
ABSTRACT
For developing the determination method of chromium picolinate in health food by HPLC,extraction method and HPLC operation conditions such as columns,mobile phase had been optimized.Long term stability test of chromium picolinate had been done.Recovery of the standard spiked >93%,the RSD was 3.1,the lowest detection limit was 10.0 mg/kg,the result fits the demand of the analysis of health food.This method can be used in analysis of the content of chromium picolinate in types of tablet and capsule health food.2 years store experiment in room temperature showed that chromium picolinate was stable.
ABSTRACT
For developing the determination method of chromium picolinate in health food by HPLC,extraction method and HPLC operation conditions such as columns,mobile phase had been optimized.Long term stability test of chromium picolinate had been done.Recovery of the standard spiked >93%,the RSD was 3.1,the lowest detection limit was 10.0 mg/kg,the result fits the demand of the analysis of health food.This method can be used in analysis of the content of chromium picolinate in types of tablet and capsule health food.2 years store experiment in room temperature showed that chromium picolinate was stable.
ABSTRACT
For developing the determination method of chromium picolinate in health food by HPLC,extraction method and HPLC operation conditions such as columns,mobile phase had been optimized.Long term stability test of chromium picolinate had been done.Recovery of the standard spiked >93%,the RSD was 3.1,the lowest detection limit was 10.0 mg/kg,the result fits the demand of the analysis of health food.This method can be used in analysis of the content of chromium picolinate in types of tablet and capsule health food.2 years store experiment in room temperature showed that chromium picolinate was stable.