ABSTRACT
A simple in vivo bioassay suitable testing of quality control of recombinant human erythropoietin (rHu-EPO) analogues was developed. Mice made polycythemic by intraperitoneal injection of 1.2 ml of a 80 per cent suspension of heterologous (rat) red cells were used as assay animals and splenic 59 Fe uptke as expression of the response to rHu-EPO. The assay took three days and the following schedule is propose: 1)intraperitoneal injection of 1.2 ml of washed packed red cells obtained from donor rats, 2) subcutaneous injection of test material 4-5 h after transfusion, 3) intravenous administration of 59 Fe tracer 48 h later, and 4) determination of splenic isotope uptake 6 h after injection. This method for the in vivo biossay of rHu-EPO analogues is an economical and reliable alternative to the existing bioassays of the hormone
Subject(s)
Animals , Mice , Rats , Female , Erythrocyte Transfusion , Erythropoietin/analysis , Polycythemia/metabolism , Biological Assay , Erythropoiesis , Iron Radioisotopes , Polycythemia/etiology , Radioactivity , Rats, WistarABSTRACT
Se presenta un paciente de 69 años de edad con cuadro clínico e histopatológico compatible con piodermitis vegetante. Las lesiones de 2 años de evolución se localizan en extremidades superiores. En los exámenes bacterilógicos por puncón se aisló en dos oportunidades Stafilococus aureus. Se destaca la buena, pero lenta respuesta a la terapéutica antimicrobiana
Subject(s)
Humans , Male , Aged , Amoxicillin-Potassium Clavulanate Combination/therapeutic use , Extremities/microbiology , Skin Diseases/diagnosis , Skin Diseases/enzymology , Skin Diseases/therapy , Staphylococcus aureus/isolation & purification , Arm/pathologyABSTRACT
Os autores analisaram as complicaçöes e seqüelas das fraturas articulares do calcâneo em 42 fraturas tratadas por diferentes métodos, tanto cruentos quanto incruentos. Os critérios de avaliaçäo foram clínicos e radiográficos. Verificaram o seguinte: a) a infecçäo superficial foi a complicaçäo mais freqüentemente encontrada; b) a dor foi a seqüela clínica mais freqüente em todos os métodos usados; c) a artrose da articulaçäo subtalar foi a seqüela que esteve presente na maioria dos métodos empregados; d) o método incruento foi o que apresentou maior número de seqüelas, tanto clínicas como radiográficas.
Subject(s)
Humans , Male , Female , Adolescent , Adult , Middle Aged , Tarsal Joints/injuries , Calcaneus/injuries , Fractures, Bone/complicationsABSTRACT
In 1985 a study was undertaken of the prevalence of Trypanosoma cruzi antibodies in 13 departments of Uruguay where transmission of the parasite by the vector Triatoma infestans persists. A total of 5 924 serum samples were selected using a probabilistic method- 3 840 from individuals over the age of 12 (sample I) and 2 084 from subjects who where 12 years old (sample II). The population was classified acording to place of residence (capital city, non-capital city, suburban área, and rural área). The percentage of positive sera detected by indirect immunofluorescence in the different departments ranged from 1 to 11 percent, and overall seroprevalence for the área was 3.4 percent. Based on the results obtained, it was possible to distinguish three áreas: A, which seroprevalence from 6 to 11 percent; B, 2 to 3.2 percent, and C, 1 to 4 percent. In sample II from the Departments of Paysandu, Soriano, Flores, Florida, and Durazno, no cases of Chagas' disease were detected which suggests that there is no active transmission of T. cruzi in this age group in the área studied. The number of persons estimated to have the disease was 36 952, or 1.3 percent of the total population of Uruguay and 4 percent of the population in the área surveyed. These seroprevalence figures are similar to those recorded in the povince of Entre Rios, Argentina, and in the neighboring municipalities of Rio Grande do Sul, Brazil
Subject(s)
Chagas Disease/immunology , Trypanosoma cruzi/isolation & purification , Prevalence , UruguaySubject(s)
Humans , Male , Female , Lead/blood , Porphobilinogen Synthase , Reference Values , Porphobilinogen Synthase/analysis , SpectrophotometrySubject(s)
Child , Adolescent , Adult , Middle Aged , Humans , Chagas Disease/epidemiology , UruguayABSTRACT
O estudo de 4.703 recem-nascidos da Unidade Neonatal do Hospital Israelita Albert Einstein, Sao Paulo, no periodo de julho de 1979 a dezembro de 1980, revelou uma maior incidencia de ictericia nos nascimentos por cesarea, nao havendo diferenca estatisticamente significativa na incidencia de ictericia entre os recem-nascidos a termo, prematuros e com desnutricao intra-uterina. Nao se evidenciou a importancia de processos patologicos neonatais (disturbios respiratorios, metabolicos e infecciosos) como fatores responsaveis por uma maior incidencia de ictericia nesse periodo de vida