ABSTRACT
The therapeutic action of phosphorylated mannanoligosaccharides (MOS) was investigated regarding its prebiotic activity on enteropathogenic Escherichia coli (EPEC). Diarrhea was induced in dogs by experimental infection with EPEC strains. Then MOS was supplied once a day, in water for 20 days. Immunological (IgA and IgG), hematological (lymphocytes, neutrophils and monocytes) and bacteriological variables (PCR detection of the eae gene of EPEC recovered from stool culture), as well as occurrence of diarrhea were evaluated. All strains caused diarrhea at 24, 48 and 72 h after infection. PCR results indicated that E. coli isolated from stool culture of all infected animals had the eae gene. There was no significant difference among groups as to number of blood cells in the hemogram and IgA and IgG production. MOS was effective in recovering of EPEC-infected dogs since prebiotic-treated animals recovered more rapidly from infection than untreated ones (p < 0.05). This is an important finding since diarrhea causes intense dehydration and nutrient loss. The use of prebiotics for humans and other animals with diarrhea can be an alternative for the treatment and prophylaxis of EPEC infections.
Subject(s)
Animals , Dogs , Blood/immunology , Diarrhea/microbiology , Enteropathogenic Escherichia coli/immunology , Feces , Gastrointestinal Agents/metabolism , Oligosaccharides/metabolism , Prebiotics , Antibodies, Bacterial/blood , Chemical Phenomena , Disease Models, Animal , Escherichia coli , Gastrointestinal Agents/administration & dosage , Gastrointestinal Agents/chemistry , Immunoglobulin A/blood , Immunoglobulin G/blood , Leukocytes/immunology , Oligosaccharides/administration & dosage , Oligosaccharides/chemistrySubject(s)
Humans , Male , Female , Irritable Bowel Syndrome/diagnosis , Irritable Bowel Syndrome/therapy , Disease Management , Odds RatioABSTRACT
Utilizando o material clinico de duas sessoes de um paciente em psicanalise, o autor e tentado a analisar algumas correlacoes entre o sintoma apresentado - labirintite - e uma lenda mitologica. Primeiramente, na introducao, o autor discute quatro pontos sobre mitos a respeito dos quais persistem algumas duvidas, ou sejam" a) a validade de usar mitos como modelos nas pesquisas psicanalitica, sociologica e antropologica; b)se o conteudo dos mitos e realmente uma expressao livre e direta do inconsciente; c)a diferenca entre mitos e contos; d) a universalidade da linguagem mitologica. Finalmente, o autor discute o material clinico e a condensacao expressa na figura do minotauro