ABSTRACT
INTRODUCTION: Ovarian cancer is generally diagnosed at advanced stages of the disease; therefore, poor prognoses are typical. The development of tumor markers is thus of utmost importance. Prostasin is a protease that in normal tissues is highly expressed only in the prostate gland and seminal fluid. A previous study showed that prostasin is highly overexpressed in ovarian cancer cell lines. This study sought to evaluate the expression of prostasin in ovarian cancer. METHODS: Fresh tumor samples of ovarian epithelial cancer (n: 12) were analyzed for expression of prostasin mRNA (messenger ribonucleic acid) by conventional and real time quantitative PCR (polymerase chain reaction). As a standard control, a normal prostate sample was analyzed. RESULTS: Using conventional PCR, prostasin was detected in all but one sample. Using quantitative PCR, prostasin was over-expressed in all but one of the samples as compared to the control (prostate). CONCLUSIONS: These findings indicate that prostasin is overexpressed in many epithelial ovarian cancers. Further studies of prostasin as a potential biomarker for this disease are warranted.
Subject(s)
Adult , Aged , Aged, 80 and over , Female , Humans , Middle Aged , Gene Expression Regulation, Neoplastic , Mass Screening/methods , Neoplasm Proteins/blood , Ovarian Neoplasms/enzymology , Serine Endopeptidases/blood , Biomarkers, Tumor/blood , Ovarian Neoplasms/diagnosis , Pilot Projects , Reverse Transcriptase Polymerase Chain ReactionSubject(s)
Humans , Male , Middle Aged , Adenocarcinoma/blood , Antibodies, Antiphospholipid/blood , Lung Neoplasms/blood , Prostatic Neoplasms/blood , Venous Thrombosis/blood , Adenocarcinoma/immunology , Lung Neoplasms/immunology , Lung Neoplasms/secondary , Prostatic Neoplasms/immunology , Venous Thrombosis/immunologyABSTRACT
Os autores apresentaram um caso de angiolipoma epidural pela raridade dessa patologia. De localizaçao epidural torácica a lesao causava maior compressao no nível de T3-T4. Nao havia disrafismos espinhais, nem relato de uso de corticosteróides e simulava enfermidade degenerativa medular pelo tempo de evoluçao.
Subject(s)
Humans , Male , Aged , Angiolipoma , Epidural Space , Thoracic Vertebrae/surgery , Hypertension , Laminectomy , Postural Balance , Reflex, BabinskiABSTRACT
Os autores relatam um caso de um paciente de 5 meses de idade, com papiloma de plexo coróide (PPC) associado a hidrocefalia importante por super-produçao liquórica. Como decorrência do tratamento, inúmeras complicaçoes ocorreram, como hematoma subdural agudo, coleçao subdural crônica de início hipertensivo, sendo necessários vários procedimentos cirúrgicos, culminando com derivaçao ventrículo peritoneal e subduro-peritoneal.