ABSTRACT
In order to investigate further the adaptive response of moulds to ambient pH, we have measured by ELISA the pho-2-encoded Pi-repressible alkaline phosphatase synthesised by Neurospora crassa. We showed that the 74A and pho-2A strains of this mould secrete similar amounts of the pho-2-encoded enzyme irrespective of ambient pH, when both the preg and pgov genes are not functional, i.e., in strains nuc-2+ growing under Pi-starvation. This suggests that pho-2, which is responsive to Pistarvation via the action of genes nuc-2, preg, pgov and nuc-1, is not a gene responsive to ambient pH and that the differential glycosylation observed for the Pi-repressible alkaline phosphatase retained by the mycelium at pH 5.6 or secreted into the growth medium at pH 8.0 is the genetic response to ambient pH sensing in N. crassa.
Subject(s)
Alkaline Phosphatase , Clinical Enzyme Tests , Genetics, Microbial/methods , In Vitro Techniques , Neurospora crassa , Bodily Secretions/enzymology , Culture Media , Immunoenzyme Techniques/methodsABSTRACT
A tipagem molecular do genoma bacteriano, na maioria das vezes, envolve a análise de fragmentos de restriçäo do DNA cromossômico. Desde que a ribotipagem foi descrita, em 1986, tem sido amplamente utilizada para analisar relaçöes taxonômicas e/ou epidemiológicas entre os diferentes grupos de organismos. A ribotipagem usa o padräo de restriçäo do opéron de RNA ribossômico (rrn) como ferramenta epidemiológica e tem fornecido ótimos resultados para a detecçäo de polimorfismo do comprimento dos fragmentos de restriçäo (RFLPs). O número de opérons rrn da bactéria está diretamente relacionado ao potencial discriminatório da técnica, fornecendo um maior ou menor número de bandas.