ABSTRACT
OBJECTIVE: To elucidate the regulation of p38 and PKC for water channel protein AQP9 and the effect on As intake in lour kinds of mammal cells, and to investigate the regulation mechanism of AOP9 phosphorylation. METHODS: p38, PKC protein and phosphorylation levels were analyzed by Western blotting, and phosphorylation level of AQP9 was detected by immuno-precipitation. Intracellular arsenic content was determined by inductively coupled plasma mass spectrometry (ICP-MS). Experimental data were analysed by SPSS statistical software. RESULTS: p38 protein and phosphorylation levels increased significantly with time in four kinds of cells treated with NaAsO2, while no significant change had been observed in PKC protein and phosphorylation levels. AQP9 phosphorylation level was inhibited in L-02 cells when p38 activity was inhibited, and As accumulation also decreased significantly in L-02 cells. CONCLUSION: AQP9 phosphorylation has effect on As intake and rate. But the regulated mechanism may be different in different cells. p38 kinase attends partly AQP9 phosphorylation in liver normal cells L-02, PKC has no effect on AQP9 phosphorylation in all kinds cells used in this paper.
ABSTRACT
Objective To explore the folate receptor mediated (FRD) check and multi-function acetic acid white solution liquid based cervical cytology (TCT) application value in cervical cancer screening.Methods A total of 602 cases of patients was tested with FRD multi-function acetic acid white solution check,and TCT and cervical biopsy pathology examination.With the used of histopathological results as the gold standard,FRD multi-function acetic acid white analysis was compared with the TCT screening inspection results.Results For a total 602 patients with TCT screening,the positive rate was 21.8％ (131/602),including 36 cases of CIN Ⅰ level,41 cases of CIN Ⅱ level,24 cases of CIN Ⅲ level,and 30 cases of cervical invasive carcinoma.For the FRD multifunction white acetate solution screening,its positive rate was 23.8％ (143/602).No statistically significant difference was found between TCT and FRD screening (P ＞ 0.05).The missed diagnosis rate of FRD multi-function white acetate solution screening was 2.6％ in inflammation,and 21.1％ in cervical invasive cancer,and 3.8 ％ in CIN.The missed diagnosis rate of TCT screening was 7.2％ in inflammation,5.3％ in CIN Ⅰ,4.9％ in CIN Ⅱ,and 58.6％ in CIN Ⅲ]; whereas,its detection coincidence rate was 100％ in squamous cells carcinoma (SCC) and adenocarcinoma (AC).FRD multi-function acetic acid white solution screening had a sensitivity 80.92％,specificity 92.14％,positive predictive value 74.13％,and negative predictive value 95.59％.TCT examination had a sensitivity 90.84％,specificity 90.23％,positive predictive value 72.12％,and negative predictive value 97.25％.No significant difference was found between FRD and TCT methods (P ＞ 0.05).Conclusions FRD and TCT methods were both efficient in screening and evaluation for cervical lesions and cervical cancer.Because FRD method is limited in the deep tube for examination of cervical lesions; it cannot completely replace the TCT examination.However,FRD method is reliable,economic,and simple operation; it is suitable for primary hospitals census of cervical cancer