ABSTRACT
Objective The purpose of this study is to investigate the expression of the polypeptide N-acetylgalactosaminyltransferase-10 (ppGalNAc-T10) protein in lung and breast and colon cancer. Methods Immunohistochemistry was used to analyse ppGalNAc-T10 expressions in 110 lung cancer tissues, 100 breast cancer tissues and 110 colon cancer tissues. Meanwhile, different cancer has 10 cases of normal tissues as control. Results The expression of ppGalNAc-T10 in normal lung tissue was higher compared with that in lung cancer organizations, and ppGalNAc-T10 positive expression was found to be significantly associated with invasion depth of tumor (P < 0. 01). PpGalNAc-T10 protein expression in breast and colon cancer was no significantly difference than that in non-tumor breast and colon tissue. Conclusion PpGalNAc-T10 expression is a useful marker in lung cancer.
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of silencing HERC4 on the proliferation, apoptosis, and migration of cervical cancer cell line Hela and the possible molecular mechanisms.</p><p><b>METHODS</b>Three HERC4-specific small interfering RNAs (siRNAs) were transfected into Hela cells, and HERC4 expression in the cells was examined with Western blotting. CCK-8 assay, annexin V-FITC/PI assay, and wound healing assay were used to assess the effect of HERC4 silencing on the proliferation, apoptosis and migration ability of Hela cells. The expression levels of cyclin D1 and Bcl-2 in the cells were detected using Western blotting.</p><p><b>RESULTS</b>Transfection of siRNA-3 resulted in significantly decreased HERC4 protein expression (P<0.01). HERC4 silencing by siRNA-3 markedly suppressed the proliferation and migration of Hela cells, increased the apoptosis rate (P<0.01) and reduced the expression levels of cyclin D1 and Bcl-2 (P<0.01).</p><p><b>CONCLUSION</b>Silencing of HERC4 efficiently inhibits the proliferation, migration, and invasion of Hela cells in vitro, and the underlying mechanisms may involve the down-regulation of cyclin D1 and Bcl-2.</p>