ABSTRACT
In this study, we isolated and purified the extracts of the whole plant of Crotalaria sessiliflora L. by column chromatographic. Twelve compounds were isolated and identified as followings: sessiliflorin B (1), quercetin (2), kaempferol (3), soyasapogenol B (4), fernenol (5), neoechinulin A (6), ethyl 4-hydroxybenzoate (7), ethyl caffeate (8), 5,7-dihydroxychromone (9), crotadihydrofuran A (10), butesuperin B (11) and aurantiamide acetate (12). Compound 1 is a new compound, compound 3-12 were isolated from the plant for the first time.
ABSTRACT
Objective: To investigate the isoflavones constituents from the whole plant of Crotalaria sessiliflora. Methods: The compounds were isolated by column chromatography and their structures were identified by the spectral data. Results: Twenty-one isflavones were isolated from 75% ethanol extract in the whole plant of C. sessiliflora and identified as 6,7,2'-trimethoxy-4'-hydroxyl isoflavone (1), 7,4'-dihydroxy-5-methoxy isoflavone (2), daidzein (3), 2'-hydroxyl genistein (4), genistein (5), 3'-hydroxyl daidzein (6), genistein 7-O-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside (7), genistein 7-O-glucoside (8), glabrone (9), alpinumisoflavone (10), barbacarpan (11), 3'-hydroxyl genistein (12), 4',7-dimethoxy-5-hydroxyl isoflavone (13), pratensein (14), 8-O-methylreyusi (15), eurycarpin A (16), prunetin (17), formononetin (18), 2'-methylalpinumisoflavone (19), hydroxyalpinumisoflavone (20), and desmoxyphllin A (21). Conclusion: Compound 1 is a new compound named sessiliflorin A, compounds 2-3, and 6-21 are isolated from the plant for the first time.