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Objective To observe the effect ofShenmal injection on vascular endothelial function after cerebral ischemia reperfusion in rats.Methods 30 rats were randomly divided into a sham operation group, a model group and aShenmal group, with 10 in each. Except the sham operation group, animal models of cerebral ischemia reperfusion injury were established by the right internal carotid artery occlusion of middle cerebral artery. Then shenmal injection was given to theShenmal group, 2 ml/100 g Sodium Chloride was given to the other groups, once per day, for successive 7 days. Endothelin-1 (ET-1), nitric oxide (NO), nitric oxide synthase (NOS), serum high sensitivity C-reactive protein (hs-CRP), and homocysteine (Hcy) were tested. Results In Shenmal group, ET-1(126.10 ± 6.07 ng/mlvs.105.41 ± 0.09 ng/ml), hs-CRP (15.93 ± 2.79 mg/Lvs. 9.33 ± 1.32 mg/L) and Hcy (11.01 ± 0.98 μmol/Lvs. 8.13 ± 0.46 μmol/L) were decreased after the treatment, while NOS (168.87 ± 9.25 U/mlvs.242.25 ± 10.36 U/ml) and NO (84.03± 6.02vs.102.42± 5.05) were increased after the treatment with statistical difference(P<0.05).ConclusionShenmalinjection can improve NOS activity and promote the synthesis of NO, decrease the level of plasma ET-1, hs-CRP and Hcy, reduce endothelial inflammatory exudation, and prevent vascular endothelial dysfunction caused by cerebral ischemia reperfusion injury.
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ObjectiveTo evaluate the therapeutic effect of Salvia miltiorrhiza and ligustrazine combined with vinpocetine in patients with cerebral infarction.MethodsAtotal of 206 patients with cerebral infarction were randomly divided into a controlgroup and a combined treatment group according to the random number table, 103 in each group. The control group was treated with aspirin and vinpocetine, the combined treatment group received Salvia miltiorrhiza and ligustrazine injection on the basis of the control group. The serum contents of malondialdehyde (MDA) and nitric oxide (NO), as well as the serum levels of superoxide dismutase (SOD) and nitric oxide synthase (NOS) were detected before and after the treatment. The electroencephalography (EEG) amplitudes and the regional cerebral blood flow (rCBF) were recorded before and after the treatment .ResultsIn the combined treatment group, the serum content of MDA was significantly decreased (151.36 ± 11.36 mmol/Lvs.62.23 ± 4.11 mmol/L;t=74.878,P<0.01), while the serum content of NO (110.31 ± 9.51 mmol/Lvs.154.23 ± 12.21 mmol/L;t=28.801,P<0.05) and the serum levels of SOD (55.52 ± 4.47 U/mlvs.85.39 ± 7.21 U/ml;t=35.735,P<0.05) and NOS (115.21 ± 8.39 mmol/Lvs.190.12 ± 11.29 mmol/L;t=54.049,P<0.01) were significantly increased than before the treatment. The rCBF (39.39 ± 2.45 ml/100 g?minvs. 69.95 ± 4.25 ml/100 g?min;t=32.385,P<0.05) and EEG (62.41% ± 4.58%vs.97.02% ± 9.67%;t=31.012,P<0.05) in the combined treatment group were significantly increased than before the treatment. The total efficiency rate in the combined treatment group was significant higher than that in the control group (88.3% vs. 66.9%;χ2=12.343, P=0.004).ConclusionsSalvia miltiorrhiza and ligustrazine combined with vinpocetine has a beneficial therapeutic effect in patients with cerebral infarction, and it is probably associated withincreasing cerebral blood flow and enhancing antioxidant activity.
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BACKGROUND:Studies have shown that under certain conditions, mesenchymal stem cels can be induced to differentiate into hepatocytes and promote local angiogenesis to generate new capilary network and build rich colateral circulation for the purpose of improvement and treatment of liver cirrhosis. OBJECTIVE:To review the latest advances in mesenchymal stem cel transplantation for the treatment of liver cirrhosis. METHODS:PubMed, CNKI, Wanfang and VIP databases were searched using keywords of marrow stem cels, hepatic cirrhosis in the title and abstracts for articles addressing mesenchymal stem cel transplantation for liver cirrhosis published from January 2000 to December 2014. Finaly, 53 articles were reviewed. RESULTS AND CONCLUSION:Mesenchymal stem cels have self-replication and multi-directional differentiation potential, and also possess paracrine and immune functions, which are ideal seed cels used for cel replacement therapy. At present, the techniques for isolation, culture, identification andin vitroamplification of mesenchymal stem cels have been quite mature, and animal studies have confirmed that umbilical cord mesenchymal stem cels is safe and effective to treat decompensated cirrhosis, which can fundamentaly treat target organ damage and improve liver function, but there are stil many clinical issues to be resolved.
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Objective To study the synovial lesions of finger joint of rheumatoid arthritis (RA) with high frequency ultrasound ( HFUS). Methods HFUS examination of finger joints of both hands were performed in 42 patients with RA and 30 healthy volunteers. The ultrasound findings of the finger joints in RA patients included intra-articular fluid,the thickness of synovial membrane,vascular proliferation. and the blood flow characteristics. Results The intra-articular fluid was found in 256 joints and the detection rate was 42. 38% (256/840). Synovial membrane hyperplasia was found in 223 joints with the detection rate of 26.55% (223/840). The detection rate of HFUS for vascular proliferation was 6.07% (51/840). Conclusions HFUS is an easy, safe and effective method for the diagnosis of RA thus providing useful evidences for clinical diagnosis.