Determination of flavonoids in tender stem and leaf of Ampelosis prossedentata by differential spectrophotometry / 中草药

Object To develop a method for the determination of flavonoids from the tender stem and leaf of Ampelosis grossedentata (Hand. Mazz.) W. T. Wang, and for its quality control. Methods Differential spectrophotometry was used at the wavelength of (318?1) nm, while taking dihydromyricetin as index. Results The regression equation, within the range of the control concentration in 4 576～22 88 ?g/mL, was A=29 56 C+0 005 5, with a good linearity (r=0 999 8) between the absorbance and concentration. The average recovery rate was 100 8% and RSD was 1 9% (n=5). Conclusion The method is simple, reliable and easy to operate and suitable for the quality control of Chinese medicinal materials with a better reproducibility.