ABSTRACT
Aim To investigate the treatment effect of Tongxinluo(TXL)combined peripheral blood derived mesenchymal stem cells(PB-MSCs)transplantation on angiogenesis of HIF-1 /VEGF pathway and miR-21 0 in diabetic foot(DF)rats.Methods Seventy male Spra-gue-Dawley rats were fed with high-fat diet and given combined streptozotocin injection to build diabetic rat model.The femoral artery and vein of right lower ex-tremity of modeled rats were ligatured to induce DF model.The normal rats in the same batch copied is-chemia model served as control group.The modeled rats were divided into 6 groups :model group ,TXL group,Cilostazol group,PB-MSCs group,TXL com-bined PB-MSCs(T-MSCs)group and Cilostazol com-bined PB-MSCs(C-MSCs)group.After the treatment for 28 days,animal gastrocnemius muscle and serum were taken for detection,in which serum was used to detect VEGF-A and HIF-1 α by ELISA,and muscle was used to detect VEGF-A and VEGF-R2 by Western blot,and VEGF-A by RT-PCR.Results Compared with the control group,the expressions of VEGF-A, HIF-1 α,VEGF-R2 and miR-21 0 in the model group were significantly reduced(P R2 and miR-21 0 in each treatment group were signifi-cantly increased(P <0.01 ),where the expressions of VEGF-A,HIF-1 α,VEGF-R2 and miR-21 0 in the T-MSCs were higher than these in the TXL,cilostazol and PB-MSCs group(P <0.05,P <0.01 ).Conclusion TXL combined with PB-MSCs transplantation may be regulated by HIF-1 /VEGF pathway and miR-21 0,pro-moting endothelial cell proliferation,differentiation, and promoting the formation of new blood vessels.
ABSTRACT
Objective To develop a method for the pretreatment of D-101 macroporous adsorption resin(MAR).Methods The influence factors of pretreatment conditions were studied by orthogonal test according to the content of toluene and naphthalene in the eluate and its UV absorbance(254 nm).Results Solvent and temperature were two key factors to the pretreatment process.The optimized method was established.D-101 MAR was eluted by 2% NaOH for 2 bed volumes(BV) of MAR and then by ethanol for only 3.5 BV at 2 BV/h at 60 ℃.At the end of elution,the UV absorbance of ethanol eluate was about 0.4.Conclusion The operation of D-101 MAR pretreatment is easy and the cycle is short.The process has little contamination to the environment and can be monitored by a on-line UV monitor.