1.
J Biosci
;
1997 Mar; 22(2): 149-159
Article
in English
| IMSEAR
| ID: sea-161105
ABSTRACT
Yeast mitochondrial RNA polymerase was purified and resolved into 2 distinct fractions. Peak A was found to be nonspecific and exhibited characteristics of the core polymerase, whereas peak Β exhibited characteristics of the holoenzyme. In vitro replication assays were carried out, using the peak Β enzyme, the cloned ori sequences and other DNA templates. It was found that ori 2 was the most efficient template for RNA polymerase primed DNA synthesis, as compared to all the other templates studied.