ABSTRACT
Objectives: To achieve certain link between diabetes, inflammation and oxidative stress, and to evaluate the effects of oral anti-diabetic agents; glibenclamide, nateglinide and Gymnema. sylvestre, inflammation and oxidative in streptozotocin [STZ]-induced diabeticc rats
Setting: Faculty of Pharmacy, Suez Canal University
Design: Group comparative study
Animals: STZ-diabetic adult male albino rats were allocated into groups and treated according to the following schedule [1] glibenclamide [2] nateglinide was used at two doses 50 ug/g body weight and 100 ug/g body weight [3] Gymnema sylvestre at two doses 200 mg/kg and 400 night; A normal control group and STZ-diabetic one were used for comparison. Intervention: Experimental diabetes was induced in rats by a single IP injection of strcptozotocin [STZ] at a dose of 45 mg/kg
Methods: Serum glucose was estimated enzymatically, hemoglobin A1C assayed by colon'mwic determination. The assay of pancreatic malondialdehyde [MDA] level, superoxide dismutasc activity [SOD] and catalase activity [CAT] were detected by colon'metric methods. Monocytc chemoattractant protein-l [MCP-l], pancreatic ihsulin content and serum insulin were estimated by ELISA. Parts of the pancreas were subjected for histopathological studies
Results: Injection of STZ induced significant hyperglycemia, which confirmed by a significant increase in serum glucose level and hemoglobin A1: and a significant decrease in serum insulin. Diabetic control fats showed significant reduction in cellular antioxidant defense system. Antioxidant enzzymes SOD and catalase activities were reduced significantly in pancreatic homogenates, while pancreatic tissue MDA level was significantly increased. STZ-induccd diabetic rats demonstrated significant increase in MCP-l. Glibenclamide induced a signihcam hypoglycemic effect and significant increase in pancreatic SOD and catalase activities. Meanwhile: nateglinide administration induced significant hypoglycemic effect associated with non significant changes in the other tested parameter. Gymnema sylvestre exhibited Significant hypoglycemic effect associated with antioxidant effect. Conclusion: Our findings indicate that the effect of natiglinide is limited as a hypoglycemic agent without antioxidant or anti-inflammatory effect. While glibenclamide and Gymnema sylvestre possess antioxidant effect in addition to their anti-diabetic activity, however they do not have effect on MCP-l
ABSTRACT
Objectives: To investigate the effect of high fat diet on inflammatory markers monocyte chemoattractant protein-1 [MCP-1] and C-reactive protein [CRP] in rats. And to investigate changes in these markers during weight reduction with diet control and orlistat treatment
Setting: Biochemistry Department, Faculty of Pharmacy and Faculty of Medicine, Suez Canal University
Design: Case control study
Materials and Methods: This study was conducted on sixty female albino rats, 15 rats received normal diet for three months and act as control. Remaining rats received high fat diet for three months and divided equally into three groups, one group continue on high fat diet, second group returned to normal diet and the third group returned to normal diet and treated with orlistat for two months. Body weight, scrum lipid profile, plasma free fatty acids [FFA] were assessed by High performance liquid chromatography [HPLC]. MCP-1 and C-reactive protein were evaluated using ELISA. Results: Feeding with high fat diet for 3 months induced significant increase in body weight [247.0 +/- 14.66] compared to control group [126. 37 +/- 23.26]. Serum total cholesterol [TC], triacylglyceroles [TAG], FFA , MCP-1 and CRP were significantly elevated in obese compared to control rats. Reduction of body weight by diet control and tetrahydrolipstatin [orlistat] treatment successfully reduced the level of MCP-1 and CRP
Conclusion: Our data showed that obesity increases chemokine levels and inflammation. Intervention to reduce these markers may help in avoidance of obesity related complications