Pharmacognostic studies on leaflets of soymida febrifuga adr. juss. family: meliaceac

Soymida febrifuga Adr. Juss. belonging to family Meliaceae, is lofty deciduous tree, found on dry stony hills and on laterite soil. The leaflets were collected from Rahuri [Ahemadnagar Dist., Maharastra]. The leaflets were studied for macroscopy, microscopy, powder characteristics, loss on drying and ash values. Microscopic studies revealed dorsiventral character of leaflets. Differentiable features like absence of stomata in upper lamina, presence of more amount of cluster type calcium oxalate crystals and presence of cicatrix were observed. Cicatrix is very rare character which can serve as identifying character. Acid soluble ash value obtained was high because of these abundant calcium oxalate crystals. Successive extractive values were determined using petroleum ether [60.80°C], chloroform and methanol. Methanolic extractive value was 26.459% w/w which is quite high. Preliminary chemical screening of extracts showed that petroleum ether extract contains sterols and triterpene type compounds while methanolic extract contains sugars, tannins, flavonols and glycosides. TLC of petroleum ether extract in benzene shows maximum 7 spots while methanolic extract in Ethyl acetate: Ethyl methyl ketone: Formic acid: water [5:3:1:1] shows maximum 6 spots

Quantitative estimation of betasitosterol, lupeol, quercetin and quercetin glycosides from leaflets of Soymida febrifuga using HPTLC technique

Soymida febrifuga [Meliaceae] dried leaflets [10 gm] were extracted with petroleum ether. Unsaponifiable matter quantitatively used for sample preparation, labeled as SF-U. Another 10 gm leaflet powder was extracted with methanol and quantitatively used for sample preparation labeled as SF-A. Sample and standard solution were dosage on three different plates and developed in its respective mobile phase plates were scanned using TLC scanner III and estimated using integration software CATs 4.05. Calculations for percentage were done considering standard and sample Rf, AUC and dilution factor. Estimation of b Sitosterol, Lupeol, Quercetin, Quercetin-3-O-galactoside, Quercetin-3-O-xyloside and Quercetin-3-O-rutinoside were determined as 0.02146% w/w, 0.0377% w/w, 0.4079% w/w, 0.6197% w/w, 2.974% w/w and 3.235% w/w respectively with the help of HPLC techniques

Isolation and characterization of lupeol and beta-sitosterol from leaves of kigelia pinnata [bignoniaceae]

Triterpene and sterol components were isolated from unsaponifiable matter of petroleum ether extract of Kigelia pinnata leaves [family: Bignoniaceae] by column chromatography and were characterized by spectral and elemental analysis. These were identified as Lupeol and beta-sitosterol. These two components were first time reported from the leaf of this plant

Isolation and characterization of lupeol and p-sitosterol from leaves of kigelia pinnata [bignoniaceae]

Triterpene and sterol components were isolated from unsaponifiable matter of petroleum ether extract of Kigelia pinnata leaves, N.O. family Bignoniaceae by column chromatography and were characterized by spectral and elemental analysis. These were identified as Lupeol and beta-sitosterol These two components were first time reported from the leaf of this plant