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ObjectiveTo investigate the potential pharmacological mechanism of Xinmaikang tablets in the treatment of atherosclerosis cardiovascular disease by using network pharmacology and cell experimental validation. MethodThe components of Xinmaikang tablets were searched by BATMAN-TCM database and the active ingredients and potential targets were screened. The atherosclerosis related disease targets were searched in GeneCards and online mendelian inheritance in man（OMIM） disease databases. The therapeutic targets were obtained by mapping the intersection of the tablets and disease targets. Therapeutic targets were uploaded to STRING database to construct protein-protein interaction（PPI） network. Cytoscape software was used to create a "drug-active component-therapeutic target" network map， and a network topology algorithm was used to screen key action targets. David software was used for gene ontology（GO） and Kyoto encyclopedia of genes and genomes（KEGG） function enrichment analysis. The key targets of drug therapy were validated by in vitro cell assay. ResultA total of 19 active ingredients， 132 potential targets and 4703 atherosclerotic disease-related target genes of Xinmaikang tablets were retrieved and screened， and 84 intersection targets were obtained. 3 key therapeutic targets of Xinmaikang tablets in the treatment of atherosclerotic diseases were screened， including Calmodulin 1（CALM1）， voltage-dependent L-type calcium channel subunit alpha-1C（CACNA1C） and Phosphatidylinositol 4，5-bisphosphate 3-kinase catalytic subunit alpha isoform（PIK3CA）. A total of 313 biological processes， 89 molecular functions and 53 cell components were obtained by GO enrichment. A total of 40 pathways were obtained from KEGG functional enrichment， including purine metabolism， renin secretion， CGMP/PKG signaling pathway and so on. In vitro cell experiment results verified that Xinmaikang tablets can up-regulate the expression of CALM1 and CACNA1C， down-regulate the expression of PIK3CA， so as to inhibit the activity of inflammatory response， and play a therapeutic role in atherosclerotic diseases. ConclusionXinmaikang tablets may treat atherosclerosis cardiovascular disease through betulin， methyleugenol and other compounds， through purine metabolism， renin secretion， cGMP/PKG signaling pathway and other pathways， which acts on CALM1， CACNA1C， PIK3CA and other targets.
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Objective:To analyze the material basis, target and pathway of Chebulae fructus and Margarita in Mongolian medicine Garidi-13 Pill on stroke with the method of network pharmacology and molecular docking technology, so as to better understand its "detoxification" mechanism. Methods:TCMIP and BATMAN-TCM were used to predict the target of Chebulae fructus and Margarita composition, and GeneCards were used to search for the target of stroke. The overlapping targets of the two platforms were imported into the Metascape software for GO biological analysis and KEGG pathway analysis. The molecular docking of key molecules and targets was carried out with LEDOCK. Results:A total of 22 active components were collected and 217 targets related to stroke were predicted. Among them, 1-O-galloyl-glucose, cuprum, ellagicacid, arjungenin and corilagin were the key substances playing the role of "detoxification" of the Chebulae fructus and Margarita; IL6, TNF, HSP90AA1, PTGS2, CASP3, NR1I2, VKORC1 and ATP1A1 were the key targets playing the role of "detoxification" . These targets were significantly enriched in cell response, humoral level regulation, hemostasis, response to steroid hormones, steroid metabolism, coagulation and other biological processes, as well as nitrogen metabolism, IL-17 signaling pathway and other pathways. Molecular docking verified the accuracy of previous prediction results from computer simulation level. Conclusion:The process of Chebulae fructus and Margarita intervening strok is closely related to the elimination of harmful metabolites and calmingthe inflammatory reaction, which was not only consistent with the modern medicine on the pathological process of stroke, but also consistent with the interpretation of "evil and poison" with Mongolian medicine theory.
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Objective To discuss the feasibility of Mongolian medicine pulse diagnosis in assessment of the disease situation of acute critically ill patients. Methods The clinical data of acute critically ill patients admitted in the Department of Emergency of Inner Mongolia International Mongolian Medical Hospital from December 2015 to December 2017 were retrospectively analyzed, and the role of Mongolian medicine pulse diagnosis in the evaluation of disease situations of patients with acute critical illness was observed. Results The improved early warning score (MEWS) at admission was carried out for all the patients in the Emergency Department of this hospital, 996 critically ill patients with their MEWS score ≥ 5 scores in accord with the inclusion criteria were allowed to undergo the Mongolian medicine pulse diagnosis, and of them, 654 patients showed specific changes in pulse condition. Shock appeared rapid pulse, microphygmia in pulse diagnosis of Mongolian medicine, severe anemia appeared the slippery pulse and rapid pulse in pulse diagnosis of Mongolian medicine, severe arrhythmia appeared rapid pulse and slow pulse in pulse diagnosis of Mongolian medicine, acute myocardial infarction appeared rapid pulse, weak pulse and slow pulse in pulse diagnosis of Mongolian medicine, insufficiency of heart function appeared rapid pulse and weak pulse in pulse diagnosis of Mongolian medicine, hypertension complicated with cerebral hemorrhage with forceful pulse and pulsus pulse are all suggestive of critical condition. Conclusions The specific changes in pulse condition of patients with acute critical illness in Mongolian medicine are helpful to the assessment of the patient's condition. This method can also be used as one of the tools for rapid assessment of disease situation in critical patients before going to hospital or in the hospital.
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Mongolian folk medicine resource is the origin of Mongolian medicine development, even more important of which is the specialized Mongolian folk medicine resources with regional and high medicine quality, it processes distinctive national characteristics with irreplaceable important position in traditional Mongolian medicine. Nevertheless, due to the serious destroy of ecological environment and sharp increase of demands, etc. A lot of specialized Mongolian folk medicine resources were endangered, and there still existed some problems in the protection and exploitation and utilization. This paper intends to provide comprehensive insight into the species protection and exploitation and utilization states of specialized Mongolian folk medicine resources. The application and protection status and the existing problems were reviewed, and the development strategies of Mongolian folk medicine resource were analyzed.
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Conservation of Natural Resources , Methods , Environment , Medicine, Mongolian Traditional , Mongolia , Plants, Medicinal , ClassificationABSTRACT
<p><b>OBJECTIVE</b>To study the analgesic, antipyretic and anti-inflammatory effect of Bailian Caogen granule.</p><p><b>METHOD</b>The antipyretic effects of Bailian Caogen granule was evaluated in rabbit fever model induced by peptone. The analgesic effect of the drug was studied with pain model of mice induced by acetic acid and hot plate, The severity of oedema in inflamed animal was observed to study the anti-inflammatory effects of Bailian Caogen granule.</p><p><b>RESULT</b>Bailian Caogen granule could obviously inhibit the fever of rabbits. The number of writhing induced by acetic acid was reduced and the pain threshold of mice was increased by Bailian Caogen granule. Bailian Caogen granule also had anti-inflammatory activity against xylene-induced mouse ear swelling and carrageenin-induced rat paw edema.</p><p><b>CONCLUSION</b>Bailian Caogen granule has significant analgesic, antipyretic and anti-inflammatory activities.</p>