ABSTRACT
Objective:To explore the diagnostic value of fine needle aspiration cytological biopsy(FNAB)under ultrasound-guided combined with the detection of point mutation of mutant B1 gene of carcinogenic homologue of murine sarcomatous virulent bacteria V600E for grade 4 nodule of thyroid imaging reporting and data system(TI-RADS)of thyroid.Methods:A total of 78 patients with TI-RADS grade 4 nodules who underwent surgery in General Hospital of Huainan Oriental Hospital Group during September 2019 and September 2021 were observed.All patients underwent ultrasound-guided FNAB examination and BRAF V600E gene testing before surgery.The postoperatively pathological results were used as gold standard to analyze the values of ultrasound-guided FNAB examination,BRAF V600E gene testing and the combined diagnosis of them.Results:Postoperatively pathological results showed 60 cases(76.92%)were malignant tumors,and all of which were papillary thyroid carcinoma.The results of Kappa consistency test between ultrasound-guided FNAB examination and pathological control,and between BRAF V600E gene testing and pathological control indicated that the Kappa values of them were respectively 0.430 and 0.583,and the Kappa value of the combined examination of them was 0.720.The sensitivity,specificity,positive predictive value and negative predictive value of the combined examination were respectively 98.33%,66.67%,90.77%and 92.31%in diagnosing thyroid nodule.Conclusion:For TI-RADS grade 4 thyroid nodule,the ultrasound-guided FNAB examination combined with BRAF V600E gene testing can significantly improve the diagnostic value.
ABSTRACT
Undifferentiated and differentiated 3T3-L1 adipocytes were treated with 100 ng/ml tumor necrosis factor-?(TNF-?),and peroxisome proliferator-activated receptor-?2 (PPAR-?2) mRNA expression and adiponectin secretion in cultured cells were measured.The results showed that TNF-?suppressed PPAR-?2 mRNA expression and adiponeetin secretion in 3T3-L1 adipocytes (P