Effect of ginsenosides on level of sex hormone receptors in human liver cell line HL-7702 / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To investigate the effect of ginsenoside (GSS) in regulating level of sex hormone receptors in human liver cell line HL-7702.</p><p><b>METHODS</b>The growth of HL-7702 were detected by MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay for choosing the available concentration of GSS; and the effect of GSS on sex hormone receptors in HL-7702 cells were detected by immuno-histochemistry, Western blot and RT-PCR.</p><p><b>RESULTS</b>GSS significantly enhance the protein and mRNA expressions of estrogen receptor (ER), progesterone receptor (PR), androgen receptor (AR) in HL7702 cell in a dose-dependent manner, the levels of expressions in the GSS treated group were higher than those in the solvent control group respectively (P < 0.05).</p><p><b>CONCLUSION</b>GSS can up-regulate the protein and mRNA expressions of sex hormone receptors in HL-7702.</p>

Experimental study on the survival of venous flap with different pedicle styles / 中华整形外科杂志

<p><b>OBJECTIVE</b>To study a new method of venous flap that is improved on its persistence and quality.</p><p><b>METHODS</b>New Zealand white rabbits were subdivided randomly into 4 groups. All rabbits were operated by harvesting a flap from the latero-abdominal wall and then sutured it in the original position. Group A: the superficial epigastric vein in the pedicle was left open (only one inflow vein remained). Group B: the pedicle vein of the proximal and distant end were left open (keeping an inflow vein and a principal out). Group C: the pedicle vein and a tributary vein were left open (keeping an inflow vein and a tributary outflow vein). Group D: the pedicle vein and two tributary veins were left open (keeping an inflow vein and two tributary outflow veins). Survival rate, MDA of the tissue, histology and ultra-microstructure were examined.</p><p><b>RESULTS</b>Survival rate of A, B, C, D were improved in order. Statistic difference is significant (P < 0.05) between group and group other than C and D. The content of MDA was heightened with statistically significant differences (A > B > C > D) among the four groups 8 hours postoperatively, but fell back to the normal level in group D and C and kept a high level in group A and B at 72 hour postoperatively. Histology and ultra-microstructure exam showed that degeneration of collagen fiber and karyopyknosis of cell is more obvious in Group A and Group B than Group C and Group D.</p><p><b>CONCLUSIONS</b>The higher survival rate of venous flap is possible by designing the more reasonable venous flap outputs pedicles which can alleviate the high tension dropsy and maintain the valid equilibrium of pour with flow in the venous flap.</p>