ABSTRACT
Background: Antibiotics are frequently used to treat acne patients either as bactericidal or anti-inflammatory agents. However, with the increased use of antibiotics, resistant strains of Propionibacterium acnes began to emerge and have been associated with a poor treatment outcome
Objectives: Detection of staphylococcal and Propionibacterium acnes strains in cases of acne vulgaris in Assiut university hospitals, Egypt and antibiotic susceptibility patterns of Propionibacterium acnes isolates
Methodology: Microbiological samples were obtained from one hundred patients with inflammatory acne lesions. Samples were cultured on blood agar and mannitol salt agar media under aerobic conditions at 37[degree]c for isolation of staphylococcal strains, and on blood agar under anaerobic conditions at 37[degree]c for 3 to 7 days for isolation of Propionibacterium acnes. Bacteria were identified by colonial morphology, standard biochemical tests, and API 20A test for identification of Propionibacterium acnes isolates. Antibiotic sensitivity testing of Propionibacterium acnes strains was done against clindamycin, erythromycin, doxycyclin, trimethoprime/sulfamethxazole, tetracycline and levofloxacin
Results: Staphylocoocal strains were detected in 55% of acne cases, while Propionibacterium acnes were detected in 35% of cases. Most Propionibacterium acnes isolates were sensitive to levofloxacin [80%], followed by doxycycline [51.4%], tetracycline, trimethoprime/ sulfamethaxazole [20.0% for each] while showed highest resistance rates to clindamycin [85.7%] and erythromycin [82.9%]
Conclusion: Levofloxacin was the most effective antibiotic for Propionibacterium acnes followed by doxycycline, while Erythromycin and clindamycin were the least effective antibiotics for Propionibacterium acnes
Subject(s)
Humans , Propionibacterium acnes/isolation & purification , Anti-Bacterial Agents , Drug Resistance, Microbial , Levofloxacin , Doxycycline , TetracyclineABSTRACT
Introduction: Vitiligo patients resistant to medical therapy pose a therapeutic problem to physicians. There are various surgical procedures for correcting stable vitiligo
Aim of work: To evaluate the efficacy of small [2-mm] punches minigraft therapy in relation to the patient age, disease site, subtype and the duration of stability of vitiligo
Patients and Methods: We used disposable 2.0-mm punch apparatus to perform minigraft therapy in 50 patients with different age range groups. Patients had either geheralized, segmental or local vitiligo, at variable sites. The duration of stability of vitiligo was different among studied patients
Results: Grading of repigmentation was assessed in relation to the age range with better results obtained in younger population [<20 years]. As regards the site of lesion, the face showed the statistically significant higher percentage of excellent response [88.89%] [P<0.05]. Segmental type of vitiligo was found to yield the best results. A significant positive correlation was detected between repigmentation score and the duration of stability of vitiligo [r=0.234, P<0. 05]
Conclusion: Age <20 years old, site of vitiligo patches, segmental subtype and longer duration of stability have positive influence on results of minigraft in patients with long standing vitiligo