ABSTRACT
OBJECTIVE@#ABO genotyping for forensic identification by oligonucleotide chip.@*METHODS@#Oligonucleotide microarrays which could detect 3 different SNPs in exon 6 and exon 7 for ABO genotyping were used. Population studies on ABO was carried out in a sample of 115 unrelated Chinese Han individuals. The method was also applied to cases.@*RESULTS@#The technique could identify 6 genotypes of ABO system. According to the results of population studies, no significant deviations from Hardy-Weinberg equilibrium could be found. The observed and expected heterozygosities were 0.591 and 0.616 respectively. The polymorphic information content was 0.544. The average exclusion probabilities in buos and trios was 0.188 and 0.344 respectively. The discrimination power is 0.777.@*CONCLUSION@#The data and case application demonstrated that ABO typing by oligonucleotide probe arrays was a useful technique for paternity testing and individual identification.
Subject(s)
Female , Humans , ABO Blood-Group System/genetics , Blood Stains , DNA/blood , DNA Primers , Forensic Medicine , Genotype , Hair/chemistry , Oligonucleotide Array Sequence AnalysisABSTRACT
Objective;To discuss if automatic detecting instrument could be applied on cell count in hydrothorax or ascites instead of traditional method. Methods; 121 cases of hydrothorax or ascites specimens were collected. SysmexUF - 100 automatic urine sediment appearance, SysmexKX -21 blood cell analysis instrument and traditional manual method were used to count white blood cell and totoal cell in all samples. The data were treat with student-T test and relativity analysis. Results;There was significant difference between data collected by SysmexUF - 100 aytinatuc urine sediment appearance and traditional manual method after T test ( P
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0.05)in results between DiaSys(R/S2003)Quantitative Analyzer of Urinary Sediment and Fast Read Urinary Sediment Quantitative-counting Board. The experiments indicated that the instrument had good precision(CV0.05,r =0.999).Conclusion:DiaSys(R/S2003)Quantitative Analyzer can complete the test of urinary sediment very well.