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To investigate the effect of Xinan Fang Yisheng-Qingluo-Huoxie prescription(YSF)on OPG/RANKL pathway in rats with rheumatoid arthritis induced by complete Freund's adjuvant(CFA)and its mechanism. Methods Thirty-two male SD rats were randomly divided into normal group, AIA group, QLY group and YSF group, with 8 rats in each group. AIA rats were induced except for the normal group. QLY and YSF were administered intragastrically on the first day after the model was successful for 30 days. The body weight and AI score of rats were observed. HE staining was used to observe the histomorphological changes of the ankle joint of rats. The expression of MMP3 and P-P65 was observed by immunohistochemistry. Serum levels of TNF-α, IL-1β, OPG and RANKL were detected by ELISA. The expression of OPG and RANKL proteins in synovium of rats was detected by Western blot and RT-PCR. Results Compared with AIA model, the body weight of the rats gradually returned to normal, and inflammation score decreased. HE staining showed that the bone was well preserved, no synovial infiltration was observed after YSF administration, and MMP3 and P-P65 showed low expression. The levels of TNF-α, IL-1β and RANKL in serum were significantly down-regulated, while the levels of OPG and OPG/RANKL were significantly up-regulated. In addition, Western blot and RT-PCR showed that OPG and OPG/RANKL were highly expressed in synovium of rats, while RANKL was on the contrary. Conclusions YSF may decrease inflammatory score and expression of TNF-α and IL-1β in serum by inhibiting inflammatory response. What's more, the increase of OPG/RANKL ratio can regulate the environment of joint bone destruction, thus alleviating RA.
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0.05).P300 and amplitudes of the mismatch negativity(MMN) of group A were lower than that of group B.PL of MMN was obviously prolonged.By comparing PL of group A with thar of group B,there was obvious difference(P
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Objective To observe the therapeutic effects and assess the feasibility of radiofrequency thermo- coagulation denervation(TRD)mediated by digital subtraction angiography(DSA)for patients with lumbar facet joint syndrome(LFJS).Methods Eighty LFJS patients were randomly divided into two groups:a denervated group(groupⅠ)and a control group (groupⅡ).The lumbar facet joints of the patients in groupⅠwere treated with TRD,while those in groupⅡhad their lumbar facet joint cavities injected with the mixture of 1 mg betamethasone and 1 ml lidocaine.All patients were assessed using the Visual Analog Scale(VAS)before treatment and 30 rain,1 d, 1 w,1 m and 6 m after.Schober values were measured at 1 w,1 m and 6 m after treatment.The therapeutic effects and side effects were assessed at 6 m after treatment.Results VAS scores in groupsⅠandⅡat 30 min,1 d,1 w and 1 m after treatment were remarkably lower than before treatment.In addition,those in groupⅠ6m after treatment were also significantly lower(P＜0.01).Compared with those in the groupⅡ,VAS scores in groupⅠwere signifi- cantly decreased at 1 m and 6 m after treatment(P＜0.05 and P＜0.01,respectively).The schober values in groupⅠat 1 m and 6m were obviously increased in comparison with those in groupⅡ,while the therapeutic effects in groupⅠat 6m after treatment were better than those in groupⅡ(P＜0.01).There were no side effects,such as injuries to the nerve roots or dysfunctional dermal sensation,in either group.Conclusion TRD,mediated by DSA,has ther- apeutic effects on LFJS.Such treatment is safe,convenient,and gives long-term pain relief.