Content determination of six flavonoids in Dendrobium officinale stems from different producing areas,cultivation and processing methods by QAMS combined with dual-wavelength method / 中国中药杂志

A novel HPLC method with the quantitative analysis of multi-components by single marker( QAMS) combined with the dual-wavelength method was developed for simultaneous determination of six flavonoids in Dendrobium officinale stems from different producing areas,cultivation and processing methods to clarify the main factors contributing to the different composition of flavonoids.The separation of six flavonoids was performed on a Shiseido Capcell PAK MGⅡ C18 column( 4. 6 mm×250 mm,5 μm) using a linear gradient elution system of acetonitrile-0. 1% formic acid aqueous solution. Schaftoside,isoschaftoside,vicenin-2,and glucosylvitexin were simultaneously analyzed using rutin as a reference standard at detection wavelength of 340 nm,and naringenin was determined at290 nm. The credibility and feasibility of QAMS method were validated and the results demonstrated that no significant differences were observed as compared with the external standard method. Finally,a total of 82 batches of D. officinale samples were analyzed and principal component analysis( PCA) and discriminant analysis were applied to distinguish and compare D. officinale samples from different producing areas,cultivation and processing methods. The results showed that the total flavonoid content of D. officinale stems cultivated in the simulated wild( attached tree cultivation or attached stone cultivation) was significantly higher than that in greenhouse bed cultivation. The content of flavonoids in simulated-wild D. officinale stems was higher in Jiangxi,Guizhou,Zhejiang,and Fujian provinces,while that in greenhouse bed cultivation was higher in Fujian and Zhejiang provinces. The content of naringenin was positively correlated with processing temperature,and that of the other five flavonoids was negatively correlated with processing temperature. PCA showed that wild-simulated D. officinale and greenhouse bed-cultivated D. officinale could be roughly divided into two clusters. The samples cultivated in the greenhouse bed were divided into four categories according to the geographical habitats. Wild-simulated D. officinale samples from Guizhou gathered together,and there was no obvious rule in samples from other producing areas. The established method simplified the determination method of flavonoids in D. officinale,and could provide the basis for effective quality control,cultivation and processing of D. officinale.

The alteration and significance of retinol binding protein 4(RBP4)in breast milk of patients with gestational diabetes mellitus / 预防医学

Objective To understand the alteration of retinol binding protein 4 (RBP4)in breast milk of women with gestational diabetes mellitus (GDM)and its clinical significance.Methods Sixty pregnant women diagnosed with GDM were selected as the cases.In the case group,breastfeeding amount reached more than 80% were defined as high -breastfeeding group while less than 20% were defined as low -breastfeeding.At the same time,45 nomal pregnant women were selected as the controls.Cord blood samples were collected during the delivery.Colostrums and mature milk samples were collected after the delivery.RBP4 and insulin concentrations were measured using enzyme -linked immunosorbent assay (ELISA ) and radioimmunoassay (RIA ) respectively.Infant birth weight and weight gain during postpartum reexamination were recorded.Results The concentrations of RBP4 and insulin in cord blood of GDM group (1 4.9 ± 2.6 μg/L and 8.3 ±1 .9 μU /mL)were higher than that in control group(1 2.4 ±2.7 μg/L and 6.0 ±2.1 μU /mL,P <0.001 ).RBP4 and insulin concentrations in colostrums of GDM group (1 6.9 ±4.2 μg/L and 1 1 .3 ±3.1 μU /mL)were higher than in controls (1 3.3 ±4.5 μg/L and 9.2 ±2.8 μU /mL,P <0.01 ).While in mature milk,RBP4 concentrations of GDMgroup were higher than that in controls (1 6.1 ±4.0 μg/L vs.1 2.5 ±3.1 μg/L,P <0.01 ).Insulin concentrationsof two groups were not significantly different.Comparing with low -breastfeeding group,RBP4 concentrations in mature milk of high -breastfeeding group were significantly lower (P <0.01 ).Cord blood RBP4 levels were positively correlated with infant birth weight (r =0.43,P <0.01 ).At the same time,in the colostrums,RBP4 levels were positively correlated with insulin levels (r =0.45,P <0.01 )and maternal weight gain during pregnancy (r =0.37,P <0.01 ).RBP4 concentrations in mature milk of high -breastfeeding group were negatively correlated with weight gain of infants (r =-0.49,P <0.01 ).Conclusion RBP4 in breast milk may be involved in the regulation of infant growth.Breastfeeding may reduce the RBP4 levels in women with GDM.

Maternal outcomes in pregnant women with pernicious placenta previa / 浙江大学学报·医学版

<p><b>OBJECTIVE</b>To analyze the maternal outcomes of pregnant women with pernicious placenta previa (PPP).</p><p><b>METHODS</b>Clinical data of 470 patients with placenta previa admitted in Women's Hospital Zhejiang University School of Medicine from August 2012 to August 2014 were collected and retrospectively analyzed. The patients were divided into pernicious group(n=101) and non-pernicious group(n=369) according to the history of cesarean section and location of placenta attached to the uterine. The general profiles, maternal outcomes of two groups were compared.</p><p><b>RESULTS</b>The age, gravidity and rate of recurrent cavity surgery of pernicious group [(32.5 ± 4.1) y, 3.4 ± 1.2, 28.7%] were higher than those of non-pernicious group [(30.7 ± 4.5) y, 2.1 ± 1.4,13.6%] (P<0.05). The gestational age of pernicious group was (35.6 ± 2.7) weeks, less than that of non-pernicious group [(36.7 ± 2.7) weeks, P<0.001]. Rate of postpartum massive hemorrhage, rate of blood transfusion, rate of placental implantation and hysterectomy in pernicious and non-pernicious group were 29.7%, 35.6%, 27.7%, 11.9% and 8.1%, 10.8%, 5.7%, 0.8%, respectively (P<0.05). Multiple regression analysis showed that placenta accrete was significantly associated with postpartum massive hemorrhage in pernicious group (P<0.05).</p><p><b>CONCLUSION</b>The awareness of the danger of pregnant women with PPP before operation and paying more attention to antenatal care are key measures to decrease the adverse maternal outcomes of pregnant women with placenta previa.</p>

Effect of vascular endothelial growth factor on pre-eclampsia in pregnant rats / 浙江大学学报·医学版

<p><b>OBJECTIVE</b>To investigate relationship between the vascular endothelial growth factor (VEGF) and the pathogenesis of pre-eclampsia in pregnant rats.</p><p><b>METHODS</b>Pregnant rats were divided into two groups randomly. Saline solution or L-nitro arginine methyl ester (L-NAME) 125 mg/d was given subcutaneously from day 7 of gestation till establishing pre-eclampsia. Systolic blood pressure, urine protein, platelet count, and weight of pups and placenta were determined. The levels of VEGF in pregnant rats venous serum, placenta and decidual tissue from normal pregnancy and pre-eclampsia rats were detected by ELISA and immunohistochemistry, respectively.</p><p><b>RESULT</b>Pregnant rats which were given L-NAME produced physical signs similar to those of pre-eclampsia, such as increase in systolic blood pressure [(145.3 +/-4.6)mmHg] and urine protein [(814.3 +/-57.5)mg/L], and decrease in platelet count [(467.1 +/-76.3) x 10(9)/L] and weight of pups and placenta. Compared with controls, the intensity of VEGF immunostaining in trophoblast or decidual cells were significantly reduced. The serum levels of VEGF were significantly lower in pre-eclampsia group than in normal pregnancy.</p><p><b>CONCLUSION</b>Decreased serum levels of VEGF and reduced expression of VEGF in placental tissues might in part explain the pathogenesis of pre-eclampsia in pregnant rats.</p>

Relationship between serum resistin level and preeclampsia / 浙江大学学报·医学版

<p><b>OBJECTIVE</b>To investigate the alteration of serum resistin levels in normal pregnancy and preeclampsia.</p><p><b>METHODS</b>Blood samples were take from 28 normal non-pregnant women, 27 women in the 1st, 26 in the 2nd and 26 in the 3rd trimester of normal pregnancy and 25 women with preeclampsia. Serum resistin concentration was determined using ELISA method.</p><p><b>RESULT</b>Serum levels of resistin were not significantly different among non-pregnancy, the 1st and the 2nd trimester of pregnancy (P>0.05 for all). Serum resistin level was significantly elevated in the 3rd trimester of pregnancy as compared with non-pregnancy (P<0.01), the 1st (P<0.001) and the 2nd trimester of pregnancy (P<0.001). Serum resistin level was significantly lower in preeclampsia than in the 3rd trimester of normal pregnancy (P<0.001), but was comparable to that of non-pregnancy, the 1st and the 2nd trimester of pregnancy (P>0.05 for all).</p><p><b>CONCLUSION</b>The decrease of serum resistin level in the preeclampsia may provide insight into the pathogenesis of the disease.</p>

Relationship between blood lipid levels of pregnant women with glucose metabolism disorders and perinatal outcomes / 中华妇产科杂志

0.05)].Maternal serum concentrations of TG and LDL-C were significantly increased in DM group [(3.6?0.9)and(4.8?0.6)mmol/L] compared with GIGT group [(2.7?0.7)and(3.8?0.9)mmol/L] and GDM group [(2.9?0.7)and(3.7?0.8) mmol/L](P0.05).The incidence of fetal distress in the GIGT group(9.8%)was lower than that in DM group(20.2%)and GDM group(21.4%,P