ABSTRACT
To study the secondary metabolites of a marine-derived fungus Ascotricha sp. ZJ-M-5, several chromatographic methods including macroporous resin, silica gel, ODS and Sephadex LH-20 were used to isolate the compounds, and their structures were elucidated on the basis of physicochemical properties and spectroscopic methods. Ten compounds were obtained and identified as ascotrichic acid B (1), (3R)-6-hydroxymellein (2), beta-carboline (3), (22E, 24R)-ergosta-7, 22-diene-3beta, 5alpha, 6beta-triol (4), (22E, 24R)-ergosta-7, 22-diene-3beta, 5alpha, 6beta, 9alpha-tetraol (5), cyclo (Leu-Pro) (6), cyclo (Ile-Leu) (7), cyclo (Pro-Val) (8), cyclo (Pro-Gly) (9), and cyclo (Hpro-Ala) (10). Among them, compound 1 is a new 3, 4-seco-lanostane triterpenoid which has been isolated from the filamentous fungi for the first time, and compounds 2-10 are firstly isolated from Ascotricha genus.
Subject(s)
Humans , Antineoplastic Agents , Chemistry , Pharmacology , Ascomycota , Chemistry , Carbolines , Chemistry , Pharmacology , Cell Line, Tumor , Cell Proliferation , Dipeptides , Chemistry , Pharmacology , Drug Screening Assays, Antitumor , Lanosterol , Chemistry , Pharmacology , Molecular Structure , Peptides, Cyclic , Chemistry , PharmacologyABSTRACT
To study the xanthones from the leaves of Calophyllum inophyllum Linn., several chromatography methods were employed to isolate the constituents. Investigation on the CHCl3 extract led to the isolation of a new xanthone named inophyxanthone A (1) and four known compounds, which were pancixanthone A (2), gerontoxanthone B (3), jacareubin (4) and pyranojacareubin (5). Among them, compound 2 was obtained from this plant firstly, and compound 3 was obtained for the first time from this genus. The structure of inophyxanthone A (1) was identified as 1, 3, 5-trihydroxy-2-(1, 1-dimethylallyl)xanthone by spectral analysis.
Subject(s)
Calophyllum , Chemistry , Molecular Structure , Plant Leaves , Chemistry , Plants, Medicinal , Chemistry , Xanthones , ChemistryABSTRACT
<p><b>AIM</b>To study the chemical constituents from the husk of Xanthoceras sorbifolia Bunge. Repeated column chromatography on silica gel and preparative TLC were used to isolate the compounds whose structures were elucidated based on spectroscopic data (ESIMS, EIMS, 1D and 2D NMR).</p><p><b>RESULTS</b>A new alkaloid was isolated and identified as: 2-methyl-6-(2', 3', 4'-trihydroxybutyl)-pyrazine (I), along with nine known compounds: cleomiscosin D (II), naringenin (III), eriodictyol (IV), kaempferol (V), quercetin (VI), rutin (VII), 5,7-dihydroxychromone (VIII), tyrosol (IX), 1-O-methyl-myo-inositol (X).</p><p><b>CONCLUSION</b>Compound I is a new alkaloid, compounds II, IV, V, VII - X were isolated from this genus for the first time.</p>