ABSTRACT
Echinocystis lobata [Michx.] Torr. et A.Gray is a spontaneous species in the Romanian flora, lesser studied by scientific literature, but which has proved significant activities in traditional medicine. The present study is aimed to provide data on the polyphenolic compounds in the composition of the flowers of this species and to test their biological potential. Polyphenols were identified and quantified using an HPLC-MS method. Tested biological activities were the cytotoxic, anti-plasmodial and antioxidant ones. Methods used for testing the antioxidant activity were the DPPH, CUPRAC, FRAP, TEAC, EPR and SNPAC assays. Cytotoxic activity was tested on cancerous and healthy cell lines and anti-plasmodial activity was assesed on two strains of Plasmodium falciparum. Ethanolic extracts of the flowers of E. lobata proved to contain isoquercitrin, rutin, quercitrin, kaempferol, p-coumaric and ferulic acid. No cytotoxic and antiplasmodial activity was found, but antioxidant assays showed an important antioxidant capacity. The obtained results show that flowers of E. lobata are important sources of antioxidant compounds. It is the first approach of the kind on the flowers of this species and it offers a new perspective on possible sources of antioxidant compounds
ABSTRACT
The Ranunculus species are poorly known as medicinal plants. They have potential toxicity given by the ranunculin and its enzymatic degradation compounds: protoanemonin and anemonin. This paper aims to evaluate the anemonin content of four species: R. bulbosus, R. ficaria, R. sardous and R. sceleratus. The evaluation was performed by TLC and HPLC. There were evaluated two types of extracts hydroalcoholic [HA] and glycerol-ethanol [GE]. The most concentrated extract in anemonin was found to be the R. sardous aerial part HA extract: 2.66 mg/ml. The lowest anemonin content is in R. sceleratus: 0.13-0.19 mg/ml. In R. bulbosus aerial part the anemonin content is less than the used HPLC method detection limits [7.68 mg/ml]. In all cases the GE extracts are less concentrated in anemonin, being more safely for human administration
ABSTRACT
The Rosemary [Rosmarinus officinalis L.], a well-known medicinal and culinary herb, was studied to compare the terpenic profile of different extracts obtained from dry and fresh herb. There were studied the volatile oil extracted by hydro distillation from dry plant, the hydroalcoholic extracts obtained from fresh respectively dry plant and the glycerol macerate obtained from fresh plant, by GC-MS using headspace injection. The separated compounds were identified using a MS spectra library. The quantitative determination was performed by normalization respectively by calibration curve method for 1,8-cineole, alpha-pinene and D-limonene. The main separated compounds were alpha-pinene, 1,8- cineol, camphene, camphor, D-limonene and cymene. A significant difference was observed between the 4 samples volatile profiles. 1,8-cineole was found major component of the essential oil [VO-21.39%] and glycerol macerate [GM- 35.60%], while and alpha-pinene was detected as the main constituent of the two tinctures [T-46.05%; MT-31.93%]. The highest 1,8-cineol content, determined by calibration curve method, was found in the volatile oil, while the fresh plant hydroalcoholic extract was richer in alpha-pinene and D-limonene
ABSTRACT
The aim of this study was focused on the polyphenolic composition and antioxidant capacity of Genista tinctoria L. and Genistella sagittalis [L.] Gams. A qualitative and quantitative characterization of the main phenolic compounds from the extracts were carried out using a HPLC-MS method. The total polyphenolic and flavonoid content was spectrophotometrically determined. The antioxidant activity towards various radicals generated in different systems was evaluated using DPPH bleaching method, Trolox equivalent antioxidant capacity assay [TEAC] and Oxygen radical absorbance capacity [ORAC], and all indicated that G. tinctoria extract was more antioxidant than G. sagittalis extract. That was in good agreement with the total polyphenolic and flavonoidic content. Chlorogenic acid, p-coumaric acid, isoquercitrin and apigenin were identified in bothspecies. Caffeic acid, ferulic acid, hyperoside, rutin, quercitrin and luteolin were found only in G. tinctoria, while quercetin was determined in G. sagittalis
Subject(s)
Antioxidants , Polyphenols , Free Radical Scavengers , Plant Extracts , Plants, Medicinal , FabaceaeABSTRACT
The purpose of our study was to identify the phenolic substances of two varieties of Chrysanthemum balsamita [balsamita and tanacetoides] and to measure the overall antioxidant activity. The phenolic compounds were determined by HPLC. The evaluation of the polyphenolic content was performed by colorimetric analysis. The antioxidant activity was measured by three in vitro assay models: the DPPH, the silver nanoparticles antioxidant capacity [SNPAC] and EPR radical detection. Using HPLC-MS analysis, phenolic acids, flavonoids and flavonoid aglycone were detected. The highest antioxidant activity was showed by Chrysanthemum balsamita var. balsamita, while the lowest for the Chrysanthemum balsamita var. tanacetoides extract, in accord with the polyphenolic content. The results show that Chrysanthemum balsamita var. balsamita might be a source of antioxidant flavonoids, especially rutin and isoquercitrin
ABSTRACT
The purpose of this study was to compare the polyphenol profile of different Rosemary [Rosmarinus officinalis] extracts obtained from dry and fresh herb and the evaluation of their antioxidant effect. There were studied the polyphenols from hydroalcoholic extracts [1:5- dry plant:solvent] obtained from fresh respectively dry plant and the gemmotherapic extract obtained from fresh plant [1:20- dry plant: solvent]. The polyphenol profile was evaluated by UV-Vis spectrophotometry, TLC and HPLC. The total polyphenol respectively flavonoids content were determined by UV-Vis spectrophotometry. The antioxidant effect was evaluated by DPPH, ABTS, FRAP, CUPRAC and silver nanoparticle [SNP] methods. The hydroalcoholic extract obtained from fresh plant contains the highest concentration of total polyphenols, [0.601mg/ml rosmarinic acid], total flavonoids, [0.270mg/ml luteoline] and rosmarinic acid [0.350 mg/ml]. The less concentrated is the gemmotherapic extract, but also the extraction ratio is higher than the hydroalcoholic extracts. The high content in polyphenols of the mentioned hydroalcoholic extract was confirmed by highest values of antioxidant activity: 39.1ml [DPPH], 7.7 ml [ABTS], 698mM ET/100 ml [FRAP], 1947 mM ET/100 ml [CUPRAC], 4570mM ET/100 ml [SNP]. These differences in the polyphenols profiles show the importance of use the fresh plants for obtaining the good quality extracts
ABSTRACT
In the present study, six indigenous species of Lamiaceae family [Origanum vulgare L., Melissa officinalis L., Rosmarinus officinalis L., Ocimum basilicum L., Salvia officinalis L. and Hyssopus officinalis L.], have been analyzed to assess the rosmarinic acid, phenyl propane derivatives and polyphenolic contents and their antioxidant and antimicrobial potential. HPLC-MS method has been used for the analysis ofrosmarinicacid. The phenyl propane derivatives and total phenolic contents were determined using spectrophotometric method. The ethanolic extracts were screened for antioxidant activities by DPPH radical scavenging, HAPX [hemoglobin ascorbate per oxidase activity inhibition], and EPR [electron paramagnetic resonance] methods. The ethanolic extracts revealed the presence of rosmarinic acid in the largest amount in O. vulgare [12.40mg/g] and in the lowest in R. officinalis [1.33 mg/g]. O. vulgare extracts exhibited the highest antioxidant capacity, in line with the rosmarinic acid and polyphenolic contents. The antimicrobial testing showed a significant activity against L. monocytogenes, S. aureus and C. albicans for all six extracts