ABSTRACT
Tumor drug resistance is currently one of the most difficult clinical problems. There are many theories on the mechanism of tumor drug resistance, and metabolic reprogramming is one of its mechanisms. Tumor cells undergo metabolic reprogramming to meet the energy requirements of proliferation, especially the changes in the glucose metabolism. This article reviews the glucose metabolism reprogramming-related enzymes and the associated small molecule inhibitors that reverse tumor drug resistance.
ABSTRACT
Protein-protein interactions (PPI)play an indispensable role in cell growth,metabolism,aging and apoptosis. Abnormal PPI will lead to cell dysfunction and diseases. Thus,it is necessary to build new technologies for target identification. PPI technologies can reveal the function of proteins and the interaction of proteins with the other molecules at molecular level,so they are widely used in identification of drug target. In this review,applications of PPI technologies in drug target identification are introduced.
ABSTRACT
Autophagy is an important physiological process in eukaryotic cells and is contributive to normal update of cellular components.When the body is in hunger state,autophagy can not only produce amino acid and ATP through degrading proteins to provide conditions for cell survival,but also remove the damaged organelles to maintain the normal functions of cells.The abnormal functions of autophagy are closely related to the formation and development of tumors,and the degree which is too high or too low both can inhibit tumors.It has been found that autophagy is regulated by many kinds of molecules,and the results are different.So in-depth studies on the mechanisms of these autophagy-related molecules will become a starting point for further understanding the autophagy and a sally port for treating tumors.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the antiproliferation effect of cardamonin (CAR) and its possible mechanisms on human umbilical artery smooth muscle cells (HUASMCs) cultured in the mimicking insulin resistance (IR) medium.</p><p><b>METHOD</b>Proliferation of HUASMCs was assayed by MTT method. The mRNA expression of mTOR, Raptor and Rictor was detected by a real-time PCR. The expression content was calculated by Livak method using internal control of beta-actin.</p><p><b>RESULT</b>The proliferation of HUASMCs cultured in the mimicking IR medium was significantly increased. Both in normal and mimic IR culture medium, cells proliferation was inhibited by CAR (1 x 10(-5), 1 x 10(-4) mol x L(-1)). Pretreated with PD98059 and LY294002, cell proliferation induced by phosphatidic acid (PA) was inhibited, and the mRNA expression of mTOR, Raptor and Rictor was significantly decreased by CAR in the mimic IR medium.</p><p><b>CONCLUSION</b>It is implicated that antiproliferation of CAR is involved in mRNA expression decrease of mTOR and its relative protein Raptor and Rictor.</p>
Subject(s)
Humans , Adaptor Proteins, Signal Transducing , Genetics , Metabolism , Carrier Proteins , Genetics , Metabolism , Cell Proliferation , Cells, Cultured , Chalcones , Pharmacology , Gene Expression Regulation , Growth Inhibitors , Pharmacology , Myocytes, Smooth Muscle , Cell Biology , Metabolism , RNA, Messenger , Genetics , Metabolism , Rapamycin-Insensitive Companion of mTOR Protein , Regulatory-Associated Protein of mTOR , TOR Serine-Threonine Kinases , Genetics , MetabolismABSTRACT
OBJECTIVE:To promote hospital rational drug use. METHODS:A total of 1 359 ADR cases submitted to State ADR Center by our hospital from 2005 to 2007 vial network system were analyzed statistically in respect of patients' age and sex,the drugs involved,routes of administration,ADR-involved organs or systems,and turnover etc. RESULTS:Of the total 1 359 cases,25.75% were induced by antimicrobial drugs and 55.33% were induced by intravenous route. The lesions of skin and its accessories were the predominant presentation of ADR,and the majority ADR cases had a good turnover. CONCLUSION:The incidence of ADR can be reduced by strengthening the education on and monitoring of ADR as well as the sense of responsibility of medical staff.
ABSTRACT
Objective To investigate the effects and the mechanism of Danshen dripping pill(DSP,复方丹参滴丸) on the lipid metabolism in rats under insulin resistant(IR) status.Methods Twenty-four Sprague-Dawley(SD) rats were randomly divided into normal control(n=8),IR control(n=8) and DSP groups(n=8).The rats were given dexamethasone(1 mg/kg) intramuscular injection once every other day to induce the IR status in the latter two groups,and the rats in DSP group were administered intra-gastrically with DSP 0.5 g/kg dissolved in normal saline once a day in the morning at 9 o′clock,and in the mean time,the rats in the normal and IR control groups were given intra-gastrically with equal amount of 0.9% NaCl. After 8 weeks of treatment,fasting blood glucose(FBG),fasting insulin(FINS),total cholesterol(TC),triglyceride(TG),nitric oxide synthase(NOS),nitrogen monoxidum(NO),smooth muscle depth/wall thickness of thoracic aorta were detected,and serum adiponectin concentration was measured by enzyme linked immunosorbent assay(ELISA),respectively.Results After IR model was successfully established,the insulin resistance index(HOMA-IR),TC and TG were increased,the serum NOS production and NO were decreased, smooth muscle depth/wall thickness of thoracic aorta was increased significantly,and the secretion of adiponectin concentration was decreased(P
ABSTRACT
AIM:To study the relationship between the genetic polymorphism of CYP3A5 gene and the blood concentration/dose(C/D) ratio of cyclosporine combined with calcium channel blockers(CCBs) in renal transplant patients.METHODS:Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) analysis was applied to determine the genotype of CYP3A5 in 69 renal transplant patients.The blood concentration/dose(C/D) ratio of cyclosporine was compared in patients coadministrated of CCBs with different genetic polymorphism of CYP3A5.RESULTS:The cases of genotype of CYP3A51/1,CYP3A51/3 and CYP3A53/3 were 8,23 and 38,respectively.The mutation rate of CYP3A53 in renal transplant patients was 71.74%.The CsA C/D ratio of patients of CYP3A51/1 and CYP3A51/3 were 1.73 and 2.43 times higher than that in patients of CYP3A53/3.The CsA C/D ratio of patients(CYP3A51/1,CYP3A51/3) combined with CCBs had been obviously elevated(19.90?7.66 vs 36.01?8.18,34.33?7.87 vs 46.16?9.63 ng?mL-1 per mg?kg-1;P
ABSTRACT
In the view of the situation in China, some speculations and suggestions on the modernization of Chinese materia medica (CMM) are given in the present paper It is very important to select the available CMM at a low price and with the better effectiveness and lower toxicity, so as to set up a national CMM catalogue, and its prescription as well Headed by pharmaceutical enterprises, to study with a target of cultivating the main Chinese medicinal materials based on Good Agriculture Practice (GAP), guaranteeing the quality control, establishing the scheme of extraction coincident with Good Extracting Practice (GEP) based on the theory of traditional Chinese medicine To make the study on the more re liable of animal model of syndromes belonged to traditional Chinese medicine and to set up the platform for drug screening and mechanism study of CMM, the evaluating system of effect and toxicity for national laboratories and clinical trial of CMM should be developed according with Good Laboratory Practice (GLP) and Good Clinic Practice (GCP)
ABSTRACT
AIM:We investigated the effects of Danshen Dripping Pill(DSP) on the glucose metabolism in rats during the insulin resistant(IR) statue. METHODS:HOMA-IR was used to show the degree of insulin resistant and Sprague-Dawley rats were randomly divided into 5 groups,normal control,IR control,Pioglitazone(PIO),Danse Injection(DSZ) and DSP groups,and the model rats were given dexamethasone to induce the insulin resistant statue.After being treated for 8 weeks,the serum adiponectin concentration and the relative expression amount of Glut-4 mRNA in skeletal muscles were detected by ELISA and real time PCR,respectively. RESULTS:The insulin resistant model rats induced by dexamethasone had a series of characters with the high level of HOMA-IR,and the serum adiponectin concentration was decreased,the Glut-4 mRNA relative expression amount in skeletal muscle cell and its translocation on the plasma membrane were inhibited.The effects of dexamethasone were significantly reversed by PIO,DSZ and DSP,respectively(P