ABSTRACT
Objective:To explore the effects of cerebrospinal fluid contained Sijunzi Decoction on function of colon mucosa lymphocytes of ulcerative colitis in vitro. Methods: Prepared the rat cerebrospinal fluid contained Sijunzi Decoction or Mesalamine. Collected colon mucosa of the 12 healthy ( as normal group ) and 18 ulcerative colitis, then isolated and obtained lymphocytes from these samples. The lymphocytes were divided into pathological group, IL-12 group, cerebrospinal fluid group cerebrospinal fluid contained Sijunzi Decoction group, cerebrospinal fluid contained Mesalamine group. After exposing to various treatment,the proliferative capacity was measured by CCK-8,the cell cycle and the ratio of CD4+T was analyzed by flow cytometry (FCM), the content of IL-1, IL-6, TNF-α was detected by ELISA. The expression of IL-2 were detected by Western blot analysis. Results:Compared the cerebrospinal fluid contained Sijunzi Decoction group with the IL-12 group or cerebrospinal fluid group,the proliferative capacity and the percentage of CD4+T was decreased, the cells significantly arrested at G0/G1 phase, the secretory capability of IL-1, IL-6, TNF-α were obviously reduced; the expression of IL-2 was also significantly down-regulated. Conclusion:The cerebrospinal fluid contained Sijunzi Decoction could decrease the function of colon mucosa lymphocytes of ulcerative colitis,the mechanism involved in may be Sijunzi Decoction regulating the secretory capability of IL-1,IL-6 and TNF-α.
ABSTRACT
Objective_To investigate the effect of ginsenoside Rg1 on the spleen structure and function of aging rats and its relative mechanism.Methods_Forty SD rats were randomly divided into normal control group, aging model group (D-galactose 120 mg/kg,qd ×42 d), Rg1 intervention group(D-galactose 120 mg/kg,qd ×42 d and Rg1 20 mg/kg, from day 15th,qd ×28 d) and Rg1 control group.After finishing injections the spleen index was meas-ured, paraffin sections were then made to observe spleen microscopic structure.Senescence-associatedβ-Galactosi-dase( SA-β-Gal) stain was used to detect aging splenocytes.The proliferative capacity of splenocytes stimulated with Concanavalin A (ConA) was measured by CCK-8.The content of IL-2,IL-6 and advanced glycosylation end products(AGEs) was detected by ELISA.The level of ROS was analyzed by flow cytometry(FCM).Malondialde-hyde(MDA), superoxide dismutase (SOD) were detected by enzymatic assay.The expression of senescence-associ-ated protein P53,P21 and RB were detected by Western blot analysis.Results_Comparing the Rg1 intervention group with the aging model group, spleen index, splenic white pulp area proportion, the proliferative capacity of splenocytes were significantly increased (P<0.05);The secretory capability of IL-2 and IL-6, the active content of SOD were obviously increased(P<0.01);The percentage of SA-β-Gal positive splenocytes, the productions of ROS and MDA were significantly decreased (P<0.01);The production of AGEs was decreased (P<0.05);The expressions of P53,P21 and Rb were also significantly down-regulated ( P<0.01) .Conclusions_Ginsenoside Rg1 relieves injure of the spleen in aging rats induced by D-galactose.It is suggested that the mechanism may be Rg1 in-hibiting oxidative stress and down-regulating P53-P21-RB signaling pathway.