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Objective To observe the expression of P21 and CDK6 in cervical squamous cell carcinoma,and to investigate the relationship between their expression and cervical squamous cell carcinoma.Methods The expression of CDK6 and P21 in 100 cases of cervical squamous cell carcinoma,20 cases of cervical CIN lesions and 20 cases of normal cervical tissues were detected by immunohistochemical ABC method,and the relationship between them and tumor differentiation,invasion depth,lymph node metastasis and clinical stage were analyzed.Results The expression rates of P21 and CDK6 in cervical squamous cell carcinoma tissues were significantly higher than those in CIN lesions and normal cervical tissues;The low expression of P21 was associated with the depth of tumor invasion,lymph node metastasis and clinical stage (P＜0.05),and was not associated with the degree of tumor differentiation (P＞0.05);The high expression of CDK6 was associated with tumor differentiation,invasion depth,lymph node metastasis and clinical stage (P＜0.05).Conclusion The abnormal expression of P21 and CDK6 may play an important role in the occurrence and development of cervical squamous cell carcinoma,and the two may have a certain significance in the prognosis of cervical squamous cell carcinoma.
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Based on the process and challenges of Universal Health Coverage(UHC) in global,the Association of Southeast Asian Nations(ASEAN) and domestic contexts were exanined.The process of realizing UHC in the international world as a whole and separately as different health system patterns was illustrated as well as the initiatives taken by the United Nation and regional agencies.The overall process,patterns of health systems,channels of health finance and design of institutions under the framework of ASEAN 10+3 UHC Network were reviewed.Opportunities and challenges for UHC in China were discussed.
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It started with the legitimacy of commercial insurance agencies undertaking claiming services for basic medical insurances.Experiences and warnings from the international world in this area were examined and causes behind it were scrutinized,under a context of the contracting-out of public services.The domestic picture was also drew and arguments were given.A three-step strategy was suggested for China's contracting-out of claiming services for basic medical insurances.
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Objectives: To investigate the current state of pension security for rural doctors in 12 provinces those have not introduced related policies. Methods:Two counties were chosen in each province. Data was analyzed in terms of coverage, types and intention of pension security for rural doctors in these areas. Results:The study found that the proportion of rural doctors with pension security was 81. 4% in these areas. 57. 1% of rural doctors with pen-sion security had urban and rural pension insurance. Differences exist among these areas. The safeguard level is too low, and the willingness of rural doctors to attend to pension security is not strong enough. Results:The mechanism of pension security for rural doctors must be improved. These areas should implement classified guidance and syn-chronize various models to solve the problem of pension security for rural doctors.
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Objective:To establish two-dimensional electrophoresis profiles with high resolution and reproducibility from immortalized human endocervical cell(H8) and cervical cancer cell(CaSKi),and to identify the differential expressions of cytoplasmic proteins.Methods:H8 cells and CaSKi cells were incubated,the cytoplasmic proteins of H8 cells and CaSKi cells were extracted and seperated by means of immobilized pH gradient-based two-dimensional gel electrophoresis(2-DE).The differential expression proteins were analyzed by using ImageMaster 2D analysis software.Results:We obtained well-resolved,reproducible 2-DE patterns,and 8 notable differential protein spots were defined in 2-DE gels.Conclusion:Cytoplasmic proteins of cervical precancerous lesion and cervical cancer are separated by means of immobilized pH gradient-based two-dimensional gel electrophoresis(2-DE).The expression maps of proteomics in cytoplasmic proteins of H8 and CaSKi are established.There are significant differences in proteome between cytoplasmic proteins of H8 cells and CaSKi cells.These data may be valuable for differential proteomics research of cervical precancerous lesion and cervical cancer.