Current status of laparoscopic central pancreatectomy / 临床肝胆病杂志

Central pancreatectomy is an ideal surgical procedure for the treatment of benign or low-grade malignant tumors in the pancreatic neck or the proximal body of the pancreas,and it can preserve more normal pancreatic tissue in order to reduce the incidence of endocrine and exocrine insufficiency after surgery.Although some clinical studies have demonstrated the feasibility and safety of this procedure,laparoscopic central pancreatectomy was technically challenging with a few number of cases.This article reviews the current status of laparoscopic central pancreatectomy and introduces our clinical experience of laparoscopic central pancreatectomy and pancreaticojejunostomy.

Effects of siRNAs targeting CD97 immune epitopes on biological behavior in breast cancer cell line MDA-MB231 / 浙江大学学报·医学版

<p><b>OBJECTIVE</b>To investigate the effects of siRNAs targeting CD97 immune epitopes on proliferation, infiltration, apoptosis and cell cycle of breast cancer cells.</p><p><b>METHODS</b>siRNA sequences targeting CD97and CD97immune epitopes were designed according to Gene Bank NM_001025160.2 with smart siCatchsiRNA design software. CD97siRNAs were transfected into MDA-MB231 cells in which CD97 was highly expressed. Highest sensitive CD97and CD97siRNA were screened by Western blotting. Inverted microscope was used to observe the growth of CD97siRNAs-transfected MDA-MB231 cells; the proliferation activity of MDA-MB231 cells was detected by MTT method; the wound healing assay and Transwell migration test were performed to examine the migration and infiltration ability of CD97and CD97siRNA-transfected MDA-MB231 cells; the effects of CD97siRNA and CD97siRNA on cell apoptosis and cell cycle of MDA-MB231 cells were detected by TUNEL and flow cytometry.</p><p><b>RESULTS</b>The growth and proliferation activity of CD97siRNAs-transfected MDA-MB231 cells were significantly lower than those in the control groups, and such differences were more significant in CD97siRNA-transfected group (all<0.05); scratch test showed that the wound healing rate was lower in CD97siRNAs-transfected groups, especially in CD97siRNA-transfected group (all<0.05); Transwell migration showed that the number of MDA-MB231 cells crossing through chambers were less in CD97siRNAs-transfected groups, especially in CD97siRNA-transfected group (all<0.05); no significant difference in cell apoptosis was observed between CD97siRNAs-transfected groups and control groups; cell cycle detection showed that CD97siRNAs-transfected groups had less cells in G/Gphase and more cells in S phase compared with the control groups, and such effect on cell cycle was more marked in CD97siRNA-transfected group (all<0.05).</p><p><b>CONCLUSIONS</b>CD97 plays an important role in the cell growth, proliferation, migration and invasion of breast cancer MDA-MB231 cells, and compared with CD97, CD97may have more effective inhibitory effects on cellular malignant behaviors.</p>

Advances in the treatment of nanoparticles targeting to gastrointestinal tumors / 国际外科学杂志

Gastrointestinal tumors are the major disease that threaten human health.According to statistics,more than 700 000 people died every year because of gastric cancer,1/10 of all cancer mortality.Colorectal cancer incidence of malignant tumors,which ranked third,ranked fourth in cancer deaths.At now the treatment of gastrointestinal tunors mostly are operation,radiotherapy and chemnotherapy,but to spread of tumors,doctors still haven' t more better method.Nanoparticles have properties in the diagnosis and treatment of cancer,including their size-dependent properties,stability in solvent,ideal size for delivery within the body,and tunable surface chemistry for targeted delivery.Here,the author do an overview of nanoparticles in gastrointestinal tumors ablation.

Progress of antioxidant therapy in sepsis / 国际外科学杂志

Sepsis is a common acute systemic infection,severe sepsis has a high rate of mortality,and its incidence rate is rising year by year.Due to the overproduction of free radicals in sepsis,microcirculation blood is drived disorder,multiple organ function can be impaired.This review describes the role of ascorbic acid in sepsis patients,which can reverse the oxidative stress injury rapidly through an eNOS-dependent mechanism,resisting platelet adhersion,preventing capillary embolism,resevering microcirculation blood flow,so as to improve patients' survival.

Hypothesis and development of tumor pre-metastatic nich / 国际外科学杂志

Tumor invasion and metastasis are regarded as main reasons for the failure of therpy and the reason of patients death.The mechnism of tumor metastasis is still uncertain.The pre-metastatic niche hypothesis provides us with new ideas to discover the mechnism.Numerous materials are involved in the formation of the pre-metastatic niche according to this hypothesis,including bone marrow-derived cells,microvesicles,exosomes,CD44,and so on.A further research on this hypothesis helps to deeply understand the nature of metastasis and leads clinical doctors to explore novel targets for clinical diagnoses and therapies.

Postoperative peritoneal adhesion in clinical treatment / 国际外科学杂志

Peritoneal adhesion is one of the common complications of abdominal and pelvic operations,the impact of which is immense in terms of human suffering and substantial socio-economic burden.There is an urgent need for a more effective understanding of the disease process and its etiology,to evaluate the pharmacologic,mechanical,and surgical adjuvant strategies to minimize peritoneal adhesion formation.