3’,4’-Dimethoxy quercetin, a flavonol compound isolated from Kalanchoe Pinnata.

From methanol extract of Kalanchoe pinnata (Lam.) Pers. leaves, a flavonol compound has been isolated. Fractionation of the methanol extract with ethyl acetate, followed by ethyl acetate fraction purification using column chromatography method with ethyl acetate : n-hexane as mobile phase gave a yellow crystal compound (A). Further analysis using 1D- and 2D-nuclear magnetic resonance (NMR), confirmed with mass spectrometer (LC-MS), compound A identified as 3’,4’-dimethoxy quercetin.

Scopoletin, a coumarin derivative compound isolated from Macaranga gigantifolia Merr.

Scopoletin (1), a coumarin derivative compound has been isolated from methanol extract of Macaranga gigantifolia Merr. leaves using chromatography methods. Chemical structure determination carried out based on spectroscopic data and compared with a reference. Scopoletin cytotoxicity test using MTT assay method against P-388 murine leukemia cells showed strong cytotoxic activity with IC50 17.42 μg/mL.

Bioactivities Evaluation of Indonesian Mistletoes (Dendrophthoe pentandra (L.) Miq.) Leaves Extracts.

Mistletoes or benalu in bahasa Indonesia is a semi-parasitic plant that also known as medicinal plant. It used in traditional/alternative medicine such as for for cough, diabetes, hypertension, cancer, diuretic, smallpox, ulcer, skin infection and after child-birth treatment. There are many species of mistletoes in Indonesia. Dendrophthoe pentandra (L.) Miq. is one of the Indonesian mistletoes species that commonly found grew on many different species of host plant. In this paper we reported in vitro toxicity, antioxidant and antidiabetes activities of MeOH and water extracts of D. pentandra grew on four different host plants (Stelechocarpus burahol, Spondias dulcis, Annona squamosa and Camellia sinensis). Toxicity was measured using brine shrimp lethality test (BSLT). Antioxidant activity was measured using DPPH free radical scavenging assay. Antidiabetes activity was measured using -glucosidase inhibitor assay. The results show that all mistletoes extracts tested (MeOH and water extracts) were non-toxic and show significant antidiabetes activity, whereas for antioxidant activity, only MeOH extracts show significant activity. Therefore, it is suggest that D. pentandra extracts are potential source for natural antioxidant and antidiabetes compounds.