Comparative Study Of Lipid Profile Between Chronic Smokeless Tobacco Consumers/ Tobacco Chewers Vis-A-Vis Non-Consumers In Tertiary Care Teaching Hospital Of Western India

Background:Ischemic heart diseases are the leading cause of death due to non-communicable diseases in India. Tobacco consumption is well proven risk factor for ischemic heart disease. Tobacco chewing is a very common practice done in Gujarat as well as other regions of India. Nicotine is the active ingredient in tobacco which causes alteration in lipid profile over long term consumption. According to “global adult tobacco survey (2016-2017)” over 21.4% of Indian adults consume smokeless/chewable tobacco. Present study was conducted to compare the lipid profile of non-tobacco consumers vs. chronic smokeless/chewable tobacco consumers.Material And Methods:The study was conducted after taking approval of Institutional Ethics Committee. A total of 100 selected study participants (non-obese male without any history of cardiovascular diseases and diabetes and not on any lipid altering medications) were divided in to case and control arm based on the history of consumption of CSLT(chronic smokeless tobacco)for 8 years or not. After overnight fasting,blood samples of both group individuals were taken for estimation of lipid profile. Details of lipid profile along with other demographic data were recorded in predesigned case record form. Result:A significant increase in lipid profile parameters such as TC, LDL, and TG were seen in chronic tobacco chewers compared to control group. Mean total cholesterol (TC), low density lipoprotein(LDL)and serum triglycerides levels in CSLT consumers were 222 mg/dl, 148 mg/dl and 171 mg/dl respectively. These parameters were higher in CSLT consumers as compared to control group by 68mg/dl (TC), 53 mg/dl (LDL) and 66 mg/dl (TG).Conclusion:Chr onic tobacco chewing was found to be associated with alteration in all the lipid profile parameters. Altered lipid profile is the proven risk for cardiovascular ailments. Hence ahypothesis can be generated from the study that CSLT consumption is the responsible factor for cardiovascular diseases. This can be tested further on large scale studies along with differences in the type of CSLT consumption and development of cardiovascular diseases can be evaluated.

Cosmetovigilance: Current Status And Need In India

Background:Usage of cosmetic products has been increased significantly in last few decades. Their usage has increased beyond the purpose of beautification. The definition of cosmetic product varies widely amidst different countries. Increased concern of physical appearance in population throughout the globe has been taken as an advantage by the cosmetic industries. Increased usage of different personal care products for prolonged period of time leads to exposure of the human body to wide variety of chemicals which maylead to adverse effects. Heavy metals have been found in certain cosmetic products which not only lead to dermatological but systemic adverse effects too. Further to this, pharmacologically active agents have been found in anti aging products. These cosmeceutical agents may possess chemical agents which are toxic to human body. Western countries have laid certain regulatory guidelines for manufacturing, labelling and marketing of cosmetic products. Cosmetovigilance ensures appropriate and safe use of cosmetic products. In India, Drugs and Cosmetic Act governs the regulations for the cosmetic products. Need of formal adverse event reporting system as well as strict regulatory guidelines for cosmetic products are required to curtail the adverse health outcomes due to cosmetic products.

Development And Characterization Of Liposomal Formulation For Ophthalmic Delivery Of Prednisolone Acetate

Liposomal vesicular drug carriers for ocular delivery have earned a wide potential nowadays. Prednisolone Acetate liposome as ocular drug carriers have been demonstrated to be a useful mode to ameliorate bioavailability and patient abidance. Liposome was prepared by lipid extruder method. Liposome were characterized for entrapment efficiency (EE %), vesicle size, surface morphology and in vitro drug release. An ex vivo corneal permeation study was performed to determine the level of drug in the external eye tissue of goat and an ocular irritation assessment was done by Hen’s Egg Test - Chorioallantoic Membrane (HET-CAM) method. The optimized formulation of liposome had shown acceptable viscosity (1.21 ± 0.03 cps), refractive index (1.47±0.001), osmolarity (303 ± 3 mOsm) and pH measurement (7.12 ± 0.09). Liposome as drug delivery carriers were evidenced to be a anticipating impendent to enhance corneal contact and penetration as well as retention time in the eye ensuing in a prolonged action and enhanced bioavailability. The results of stability study exhibited stable profile of developed liposomes.

First reported enterovirus D68 infection in pediatric patients from the Caribbean region: evidence of spread from the U.S. outbreak / Primeros casos notificados en el Caribe de infección por enterovirus D68 en pacientes pediátricos: pruebas de la propagación del brote de los Estados Unidos

ABSTRACT The 2014 enterovirus D68 (EV-D68) outbreak in the United States raised concerns about the introduction of the virus in the Caribbean region. The objective of this study was to provide rapid evidence of the introduction of EV-D68 strains in the Caribbean region during the 2014 outbreak in the United States, using a relatively simple phylogenetic approach. From October 2014 to May 2015, four EV-D68 cases from two countries (Bermuda and Dominica) were detected at the regional referral laboratory at the Caribbean Public Health Agency (Port of Spain, Trinidad and Tobago) based on molecular testing of respiratory specimens. All cases were children presenting to hospitals with moderate respiratory distress. No cases of acute flaccid paralysis were detected. Phylogenetic analysis of the Caribbean strains showed more than 99% similarity with the 2014 U.S.-outbreak strain, providing evidence of the introduction and circulation of the virus in the region.(AU)

RESUMEN El brote de enterovirus D68 (EV-D68) registrado en el 2014 en los Estados Unidos suscitó preocupación acerca de la introducción del virus en el Caribe. El objetivo de este estudio fue aportar pruebas rápidas, mediante la adopción de un enfoque filogénico relativamente sencillo, de que durante ese brote ingresaron en el Caribe cepas del EV-D68. Entre octubre del 2014 y mayo del 2015, el laboratorio regional de referencia ubicado en el Organismo de Salud Pública del Caribe (Puerto España, Trinidad y Tabago) detectó cuatro casos de EV-D68 provenientes de dos países (Bermudas y Dominica) mediante el análisis molecular de muestras respiratorias. Todos los casos correspondían a niños que acudieron al hospital con dificultad respiratoria moderada. No se detectó ningún caso de parálisis flácida aguda. El análisis filogénico de las cepas encontradas en el Caribe demostró una semejanza superior al 99 % con la cepa responsable del brote del 2014 en los Estados Unidos, lo que demuestra la introducción y la circulación del virus en la región.(AU)

Impact of environmental temperatures on resistance to organophosphate insecticides in Aedes aegypti from Trinidad / Repercusión de las temperaturas ambientales sobre la resistencia de Aedes aegypti a los insecticidas organofosforados en Trinidad

OBJECTIVE: To examine the effects of increasing larval rearing temperatures on the resistance status of Trinidadian populations of Aedes aegypti to organophosphate (OP) insecticides. METHODS: In 2007-2008, bioassays and biochemical assays were conducted on A. aegypti larvae collected in 2006 from eight geographically distinct areas in Trinidad (Trinidad and Tobago). Larval populations were reared at four temperatures (28 ± 2ºC, 32ºC, 34ºC, and 36ºC) prior to bioassays with OP insecticides (fenthion, malathion, and temephos) and biochemical assays for esterase enzymes. RESULTS: Most larval populations reared at 28 ± 2ºC were susceptible to fenthion (>98% mortality) but resistant to malathion and temephos (< 80% mortality). A positive association was found between resistance to OP insecticides and increased activities of α- and β-esterases in larval populations reared at 28 ± 2ºC. Although larval populations reared at higher temperatures showed variations in resistance to OPs, there was a general increase in susceptibility. However, increases or decreases in activity levels of enzymes did not always correspond with an increase or decrease in the proportion of resistant individuals reared at higher temperatures. CONCLUSIONS: Although global warming may cause an increase in dengue transmission, based on the current results, the use of insecticides for dengue prevention and control may yet be effective if temperatures increase as projected.

OBJETIVO: Examinar los efectos del aumento de las temperaturas de desarrollo larvario sobre el estado de resistencia a los insecticidas organofosforados de las poblaciones de Aedes aegypti en Trinidad. MÉTODOS: En 2007 y 2008 se llevaron a cabo ensayos biológicos y bioquímicos en larvas de A. aegypti recogidas en el 2006 de ocho áreas geográficamente separadas en Trinidad (Trinidad y Tabago). Las poblaciones larvarias se desarrollaron en cuatro temperaturas (28 ± 2 ºC, 32 ºC, 34 ºC y 36 ºC) antes de los ensayos biológicos con insecticidas organofosforados (fentión, malatión y temefós) y los análisis bioquímicos para las enzimas de esterasa. RESULTADOS: La mayoría de las poblaciones larvarias que se desarrollaron a 28 ± 2 ºC fueron susceptibles al fentión (mortalidad > 98%) pero resistentes al malatión y al temefós (mortalidad < 80%). Se encontró una asociación positiva entre la resistencia a los insecticidas organofosforados y la mayor actividad de αy β-esterasas en las poblaciones larvarias que se desarrollaron a 28 ± 2 ºC. Aunque las poblaciones larvarias que se desarrollaron a temperaturas mayores mostraron variaciones en la resistencia a los organofosforados, hubo un aumento general de la sensibilidad. Sin embargo, los aumentos o las disminuciones en los niveles de actividad de las enzimas no siempre se correspondieron con un aumento o disminución en la proporción de individuos resistentes desarrollados a las temperaturas más altas. CONCLUSIONES: Aunque el recalentamiento del planeta puede causar un aumento de la transmisión del dengue, según los resultados de este estudio el uso de insecticidas para la prevención y el control del dengue todavía puede ser eficaz si las temperaturas aumentan según lo proyectado.

Dengue in Costa Rica: the gap in local scientific research

El dengue es una enfermedad de gran importancia a escala mundial. Los esfuerzos para su control en la mayoría de las regiones del mundo no han logrado detener el aumento en su incidencia, y se fomenta la investigación científica para contribuir a desarrollar nuevos enfoques de control basados en la evidencia científica o a mejorar los existentes. Enfermedad recientemente reemergente en Costa Rica, el dengue se ha convertido en un grave problema de salud en ese país. A pesar de esta crítica situación, se dispone de pocas publicaciones científicas sobre dengue en Costa Rica. En una búsqueda realizada en la base de datos PubMed se encontraron solo 11 artículos, mientras que en varias bases de datos bibliográficas centradas en Costa Rica y otros países de América Latina se hallaron 19 artículos más. Además, se encontraron 10 tesis relacionadas con el dengue realizadas en universidades costarricenses. Por lo tanto, son pocos los artículos científicos originales publicados sobre este tema en revistas científicas arbitradas, especialmente en aspectos vinculados con la epidemiología, la salud pública y la idoneidad y eficacia de las intervenciones en marcha. Esta brecha en las investigaciones puede deberse a diversos factores, como el contexto histórico y político, insuficientes recursos financieros y humanos, deficiencias en la colaboración entre las instituciones y dificultades para disponer de los datos. Costa Rica puede aprender de la experiencia de otros países de la Región de las Américas (como Cuba y Trinidad y Tobago), donde se ha investigado mucho más sobre dengue. Las investigaciones de esos dos países han aportado evidencias cruciales para el desarrollo de estrategias locales y generales dirigidas al control y la prevención del dengue. En dependencia del contexto local, algunos métodos de control pueden ser más eficaces que otros, por lo que las acciones basadas en la evidencia deben adaptarse para las diferentes regiones del mundo,...

Lymphatic filariasis in the Caribbean region: the opportunity for its elimination and certification

In order to support the case for a certification of elimination of lymphatic filariasis (LF) in some Caribbean countries, we compared the prevalence of circulating Wuchereria bancrofti antigen in communities in Guyana, Suriname, and Trinidad. For the study, we assayed school children in six communities in Guyana, five communities in Suriname, and three communities in Trinidad for the prevalence of circulating W. bancrofti antigen, using a new immunochromatographic test for LF. We also assayed adults in these three countries, with a special focus on Blanchisseuse, Trinidad, where mass treatment for LF elimination had been carried out in 1981. The prevalences of W. bancrofti circulating antigen found in the school children populations ranged from 1.7 to 33.2 in Guyana and were 0.22 overall in Suriname and 0.0 in Trinidad. Among adults in two Guyana communities the prevalences were 16.7 and 32.1. The results were all negative from 211 adults in communities in the north, center, and south of Trinidad, as well as from 29 adults in Suriname. The data suggest that contrary to reports of LF endemicity from the World Health Organization, LF may no longer be present in Trinidad and may be of very low prevalence in Suriname. Trinidad and Tobago and other Caribbean nations proven negative could seek to be awarded a certificate of LF elimination. In Suriname the small localized pocket of infected persons who may serve as a reservoir of LF infection could be tested and appropriately treated to achieve LF elimination. Such LF-positive countries as Guyana should access new international resources being made available for LF elimination efforts. An adequate certification program would help identify which countries should seek the new LF elimination resources

Synthesis and anti-HIV activity of some non-nucleoside 2,3-disubstituted quinazoline derivatives (Part-V).

Several [2-phenyl-4(3H)-oxo-3-quinazolinylamino]-N-substituted- arylacetamides (1a-j) have been synthesized and tested at the National Cancer Institute, Bethesda, Maryland, USA, for their anti-HIV activity against susceptible human host cells (CEM cell line) over a wide range of concentrations (6.35 x 10(-8) to 2.00 x 10(-4) M). The highest protection observed is 45.67%. The structures of these compounds have been established on the basis of elemental analysis and spectral data.

Imported cerebral malaria complicated with glucose 6 phosphate dehydrogenase deficiency

Two cases of cerebral malaria imported from Guyana and Ghana are reported. These are the first cases of cerebral malaria diagnosed and treated in Trinidad and Tobago since malaria was eradicated. The management of both these cases was complicated because the patients' erythrocytes were glucose-6-phosphate dehydrogenase-deficient, and by the occurrence of blackwater fever, cerebral manifestations, renal impairment, hyperglycaemia and thrombocytopenia. The symptoms of cerebral malaria resolved following treatment with quinidine and doxycycline and quinidine and clindamycin.

A case of vivax malaria presenting with psyhosis

The first case of psychosis due to Plasmodium vivax malaria, imported from India is reported. A 44-year-old Trinidadian male presented with fever, and psychotic episodes in association with vivax malaria. The symptoms of both malaria and psychosis were resolved following the standard chloroquine-primaquine therapy

Falciparum malaria treated with a chloroquine suspension administered rectally in Trinidad

A new mode of administering malaria chemotherapy to patients unable to tolerate oral medication is described. A patient with Plasmodium falciparum malaria, severe hyponatraemia and hypokalaemia who regurgitated oral treatment of chloroquine phosphate and pyrimethamine and sulfadoxine (Fansidar) is presented. But neither chloroquine nor quinine intravenous formulations were available locally. As the patient was deteriorating, a suspension of chloroquine phosphate was prepared and administered rectally, resulting in a decline in the level of parasitaemia from ++++ to ++ within 48 hours. The patient improved, and further clinical management was uncomplicated.

Entomological activities during the yellow fever epidemic in Trinidad, 1978-1980

An epidemic of sylvan yellow fever occurred in Trinidad during the period 1978 to 1980. Entomological activities were initiated at various localities where monkey (Alouatta spp.) mortalities were reported. A total of 5,468 Haemagogus janthinomys was collected and tested which resulted in the isolation of 17 strains of yellow fever virus. Two strains of the virus were isolated from a total of 49 Haemagogus leucocelaenus and these constituted the first isolations of yellow fever virus from this species of mosquito from Trinidad. Attempts to demonstrate transovarial transmission of yellow fever virus from adults feared from eggs and larvae collected in the field were unsuccessful. However, from two locations virus isolations were made at the end of rainy season (January) and at the beginning of the successive rainy season (June).

Vector control operations during a sylvatic yellow fever outbreak in Trinidad,1978-1980

An outline is given of the vector control measures adopted during the 1978-1980 yellow fever epidemic in Trinidad and the efficacy of the yellow fever plan of action is discussed.

Sylvatic yellow fever activity in Trinidad 1988-1989

Of a total of 18,068 mosquitoes (361 pools) collected in south-eastern Trinidad forests from December, 1988 to May, 1989, 47 species belonging to 14 genera were identified. Five yellow fever virus isolates were made from Haemagogus janthinomys and one from Sabethes chloropterus. All the other pools of mosquitoes examined were negative for the virus. The mosquito isolates were made in December and January. In addition, in late February and early March, two infected howler monkeys (Alouatta sp.) were detected. Since March, despite continued surveillance, no yellow fever virus has been detected in mosquitoes or monkeys. There has been no reported human infection.

Yellow fever studies in Trinidad, 1988-1989: attempts to demonstrate Transovarial Transmission of yellow fever virus in Haemagogus mosquitoes collected in nature

Subsequent to an epizootic of yellow fever (YF) in monkeys and the isolation of yellow fever (YF) virus from sylvatic mosquitoes in December, 1988 and January, 1989 in south-east Trinidad, ovitrapping for mosquito eggs was started in January, 1989, in an attempt to demonstrate transovarial transmission of the virus. Multi-paddle ovitraps were used. From a total of 52,632 eggs collected in various parts of the country, the following species of mosquitoes were reared: Haemagogus janthinomys (54 per cent ), Hg. leucocelaenus (32 per cent ), Aedes insolitus (12 per cent ), Hg. equinus (1 per cent ). When mosquito eggs were removed from the hard board paddles then vacuum-treated for hatching, the process proved very economical in storage space. Most eggs were obtained at the start of the wet season, four to five months after the isolation yellow fever virus from wild caught adult mosquitoes and monkey carcasses. When adult mosquitoes reared from eggs were processed for yellow fever virus, no virus was detected from any of the mosquito pools to indicate that transovarial transmission had occurred.

Emergency vector control of urban vectors during an epizootic of yellow fever in Trinidad: 1988-1989

Sylvatic yellow fever outbreaks in Trinidad have been known to occur since the 17th century. During 1988-89 an outbreak of yellow fever occurred with the virus being isolated from monkeys and mosquitoes. This article summarizes the vector control operations adopted to prevent urban transmission of the yellow fever virus by Aedes aegypti Linn. No human cases of yellow fever were detected either in the rural or urban areas.

Métodos de evaluación de la poblacion de Aedes Aegypti y tratamientos con insecticidas en una poblacion de Trinidad, Antillas / Methods of evaluating Aedes Aegypti populations and insecticide treatment of a population in Trinidad

Métodos de evaluación de la poblacion de Aedes Aegypti y tratamientos con insecticidas en una poblacion de Trinidad, Antillas

Prevalence of leprosy in children of leprosy parents.

A cross sectional clinical study was done in slums and adjoining village of Raipur town. All the children in 100 families, in which at least one patient of proved leprosy was present were examined. Children of 100 non-leprosy families served as control. In leprous families prevalence was 14.2 times higher in comparison to children in control group. Also prevalence was higher in children of those families in which number of patients were more than one, or there was lepromatous leprosy. In children the common type of lesion were tuberculoid, indeterminate, borderline and pure neural type in that order, while no case of lepromatous leprosy was seen.