ABSTRACT
Samples of green colonial unicellular microalga Botryococcus braunii were collected from Bear Shola Falls at Kodaikanal (latitude 10.31 N and longitude 77.32 E), Tamil Nadu, India. Specimens were isolated, cultured and examined for its hydrocarbon content, morphological features and DNA structural resemblance with the known strain to confirm its identity. Inter simple sequence repeats (ISSR) finger printing revealed strong genetic similarity among the authentic strain (B. braunii N-836) and the Indian isolated strain (B. braunii CFTRI- Bb1) from Kodaikanal. The type of hydrocarbons produced by the Kodaikanal isolates were analyzed and identified as saturated hydrocarbons in the range of C21 to C33 by GCMS. Tetracosane and octacosane were found as the major components among the saturated hydrocarbons produced by this alga, constituting 17.6 percent and 14.8 percent respectively. Hydrocarbon content of the organism was in the range of 13-18 percent of its dry biomass. The fat content of the organism was found to be 22 percent (w/w). Palmitic and oleic acids were found to be major fatty acids produced by the alga. Lutein and beta-carotene were found to be the major carotenoids and constituted about 64.1 percent and 25.1 percent respectively of the total carotenoids. Based on ISSR finger printing and hydrocarbon analyses the new algal strain from Kodaikanal was identified as Botryococcus braunii.