ABSTRACT
The objective of the present study was to formulate and evaluate Tretinoin proniosomal gel and to carry out comparative skin irritation study with conventional Tretinoin solution and Tretinoin conventional gel. Topical Tretinoin (0.25%, 0.05%) has been a reliable treatment of acne vulgaris since 30 years but its major drawback is that it causes skin irritation on the applied area. The proniosomal dispersion was prepared using different grades of non-ionic surfactants and cholesterol in different ratios along with Tretinoin. The scanning electron microscopy revealed that the proniosome vesicles were of LUV type and spherical shape. The proniosome vesicles prepared with SPAN 60, 40 and cholesterol in formulation PN9 showed maximum entrapment efficiency (76.77±1.54) .The prepared proniosome vesicles were incorporated into Carbopol gel (1%) base to prepare Tretinoin proniosomal gel. The stability study was carried out at different accelerated and non-accelerated conditions. The In-vitro diffusion study carried out using sigma dialysis membrane showed sustained release pattern of Tretinoin from proniosomal gel formulation. The comparative skin irritation study carried out on 18 healthy Wistar Rats of either sex showed remarkable decrease in signs of skin irritation caused by Tretinoin.
ABSTRACT
Despite of its effective anti-tumour activity,L-Asparaginase has limited clinical application due to the high rate of clinical hypersensitivity. In an attempt to develop a liposomal drug delivery for L-Asparaginase, enzyme loaded liposomes were formulated using soy lecithin, cholesterol and charge inducers by thin film hydration method. The effect of various components of the liposomes including the concentration of lecithin and cholesterol with or without the charge inducers on the entrapment efficiency and short term invitro cytotoxicity study was systematically investigated. The average particle sizes of the vesicles were found to be 43.2, 35.6 and 65.8 μm respectively for neutral, positive and negative liposomes. The percentage of drug loading was found to be 1.95, 2.39 and 2.35 % respectively for neutral, positive and negative liposomes.The invitro release study of L-Asparaginase was carried out using normal saline as dissolution medium and the release was found to be 86.88, 78.29 and 82.04 % respectively for neutral, positive and negative liposomes. The release of LAsparaginase from liposomes was followed first order kinetics obeying non-Fickian diffusion. A short term cytotoxicity study was carried out using Ehrlich Ascites Carcinoma cells (EAC cells) which revealed that the cytotoxicity concentration CTC50 for pure drug was found to be 64 mcg as compared to liposomal formulation of 50 mcg.