Study on the distribution of hepatitis C virus genotypes in patients visiting one methadone maintenance clinic in Wuhan / 中华流行病学杂志

<p><b>OBJECTIVE</b>To study the prevalence of hepatitis C virus (HCV) infection and characteristics on molecular biology related to HCV among patients who were enrolled in a Methadone maintenance clinic in Wuhan.</p><p><b>METHODS</b>Serum samples from 332 injection drug users (IDUs) were obtained and anti-HCV IgG was detected by enzyme linked immunosorbrent assay(ELISA), together with 86 anti-HCV positive specimens genotyped. A reverse transcriptase-polymerase chain reaction (RT-nPCR) assay using conserved primers deduced from the core-envelopel (C-E1) region of the HCV genome was employed to amplify a 474 bp fragment. Phylogenetic analysis of the C-E1 sequences was conducted by direct sequencing of the RT-nPCR products and alignment with determined by nucleotide sequencing followed by composition of a phylogenetic tree.</p><p><b>RESULTS</b>There were 313 cases (94.3%) appeared positive anti-HCV IgG in the 332 patients from a Methadone maintenance clinic in Wuhan. It was demonstrated that there were four different subtypes of HCV in that clinic in Wuhan, including 6a--71 cases (82.5%), 3b--7 cases (8.2%), 1a--5 cases (5.8%) and 1b--3 cases (3.5%).</p><p><b>CONCLUSION</b>Infection of 6a genotype HCV was predominant in patients from the Methadone maintenance clinic in Wuhan, followed by HCV 3b, 1a and 1b.</p>

Combination treatment with a predominance of hysteroscopic operations for severe intrauterine adhesion: Clinical study of 27 cases / 中国微创外科杂志

Objective To evaluate the effectiveness and safety of combination treatment with a predominance of hysteroscopic operations in the management of severe intrauterine adhesion (IUA). Methods Hysteroscopic exclusion was performed in 27 patients who were confirmed as severe fibrous IUA by hysteroscopy between April 2000 and March 2003. Results All the 27 patients underwent 58 times of hysteroscopic exclusion: once in 12 patients, twice in 7 patients, 3 times in 3 patients, 4 times in 2 patients and 5 times in 3 patients. Postoperative follow-up ranged 8～46 months (mean,27 months). Menstruation returned to normal in 65.2% of the patients (15/23), amenorrhea continued in 26.1% of the patients (6/23), and hypomenorrhea remained in 8.7% of the patients (2/23). Shape of uterus cavity returned to normal in 63.0% of the patients (17/27) and to basically normal in 33.3% of the patients (9/27). The total effective rate of the study was 96.3% (26/27) while a re-adhesion took place in 3.7% of the patients (1/27). The pregnancy rate after operation was 57.1% (4/7) and the live delivery rate 42.9% (3/7). There were no operative complications in the study. Conclusions The combination treatment with a predominance of hysteroscopic operations in the management of severe IUA is safe and effective. IUD placement and sodium hyaluronate can prevent the postoperative re-adhesion. Periodic treatment of estrogen and progestogen has some actions for repairing endometrium.