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Braz. j. med. biol. res ; 26(12): 1305-17, Dec. 1993. ilus, tab, graf
Article in English | LILACS | ID: lil-148836

ABSTRACT

1. The rabies virus (Pasteur PV strain) was propagated in VERO cells attached to microcarriers in a 3.7-1 bioreactor. Virus titers of about 10(6) LD50/ml were obtained regularly. 2. Ultrafiltration was efficient for concentrating the virus suspensions, and the sucrose gradient reduced the residual VERO cell DNA to acceptable levels (less than 50 pg/dose). The remaining cell DNA content was evaluated by dot-blot hybridization with a probe prepared with VERO cell DNA. 3. The final virus preparations were inactivated by B-propiolactone treatment, showed a potency higher than 2.5 IU/dose and protected mice experimentally infected intracerebrally with rabies virus (CVS-13.2). 4. This methodology for the production of a rabies vaccine for human use should be of interest to countries where high technology facilities are not available


Subject(s)
Humans , Animals , Antibodies, Viral/immunology , Rabies Vaccines/immunology , Rabies virus/immunology , DNA/analysis , Immunoblotting , Nucleic Acid Hybridization , Time Factors , Vaccines, Inactivated/immunology , Vero Cells , Rabies virus/growth & development
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