Regulation of ischemic stroke by circadian rhythm and intervention by traditional Chinese medicine / 中国中药杂志

Circadian rhythm is an internal regulatory mechanism formed in organisms in response to the circadian periodicity in the environment, which modulates the pathophysiological events, occurrence and development of diseases, and the response to treatment in mammals. It significantly influences the susceptibility, injury, and recovery of ischemic stroke, and the response to therapy. Accumulating evidence indicates that circadian rhythms not only regulate the important physiological factors of ischemic stroke events, such as blood pressure and coagulation-fibrinolysis system, but also participate in the immuno-inflammatory reaction mediated by glial cells and peripheral immune cells after ischemic injury and the regulation of neurovascular unit(NVU). This article aims to link molecular, cellular, and physiological pathways in circadian biology to the clinical consequences of ischemic stroke and to illustrate the impact of circadian rhythms on ischemic stroke pathogenesis, the regulation of NVU, and the immuno-inflammatory responses. The regulation of circadian rhythm by traditional Chinese medicine is reviewed, and the research progress of traditional Chinese medicine intervention in circadian rhythm is summarized to provide a reasonable and valuable reference for the follow-up traditional Chinese medicine research and molecular mechanism research of circadian rhythm.

Mechanism of Huoxue Rongluo recipe in promoting angiogenesis after ischemic stroke based on correlation between miR-370-3p and JAK2/STAT3 pathway / 中国药理学通报

Aim To explore the mechanism of Huoxue Rongluo reeipe in promoting angiogenesis after ischemic stroke based on the correlation between mir-370-3p and JAK2/STAT3 pathway.Methods Hats were randomly divided into six groups.MCAO/R method was used to establish the model.After seven days of intragastrie administration,the expressions of CD31 ,vWF and vascular endothelial growth factor ( VEGF) in brain tissue were observed by immunofluorescence stai- ning; the expression of JAK2 ,p-jak2,STAT3 and p- STAT3 in brain tissue was detected by Western blot; J the expressions of JAK2,STAT3 mRNA and mir-370- 3p in brain tissue were detected by real-time PCR f RT-PCR) ; the correlation between mir-370-3p and JAK2/STAT3 pathway was analyzed by Pearson correlation ; the expressions of lncma-hl9 and mir-370-3p were detected by real-time quantitative PCR ( RT qPCR) ; the targeting relationship between lncrna-hl9 and mir-370-3p was detected by luciferase reporter assay.Results Huoxue Rongluo decoction could increase the microvessel density and average fluorescence intensity of VEGF,up-regulate JAK2 and STAT3 mR- NA,down-regulate the expression of mir-37()-3p,and promote the expressions of JAK2,p-jak2 ,STAT3 and p- STAT3.Mir-370-3p was highly negatively correlated with JAK2 and STAT3 mRNA respectively,which could be reversed by stattic,an inhibitor of STAT3 SH2 domain.Conclusions Huoxue Rongluo recipe may stimulate angiogenesis after ischemic stroke by down- regulating the expression of mir-370-3p,activating JAK2 / STAT3 pathway and promoting the expression of downstream VEGF,so as to improve the symptoms of neurolo.

Regulatory Mechanism of Traditional Chinese Medicine in Promoting Angiogenesis After Cerebral Infarction Based on STAT3/miRNA Feedback Loop / 中国实验方剂学杂志

Cerebral infarction is a clinical disease with corresponding neurological symptoms caused by cerebral ischemia and hypoxia caused by cerebral blood supply disorder. It is one of the most common cerebrovascular diseases and a serious threat to human health. The prevention and treatment of cerebral infarction has an important social significance. Angiogenesis is the key starting point for medical treatment of cerebral infarction, and signal transduction and transcriptional activators (STAT)/hypoxia inducing factor-1(HIF-1)/vascular endothelial growth factor (VEGF) pathway are important pathways to mediate angiogenesis after cerebral infarction. This paper took the angiogenesis as the starting point and the upstream molecules of STAT/HIF-1/VEGF signal pathway STAT3 and miRNA as the main study objects, and comprehensively discussed the results of chip sequencing, experimental research, traditional Chinese medicine (TCM) pathogenesis and TCM treatment. Based on the regulatory mode of "TF-miRNA" and the idea of "micro-whole", it is suggested that under the guidance of the basic theory of TCM, cubic compound prescriptions of TCM and its active components might activate the STAT/HIF-1/VEGF signal pathway through STAT3/miRNA feedback loop to promote angiogenesis after cerebral infarction, which puts forward a deep molecular mechanism and new direction for the treatment of cerebral infarction with TCM.

Effects of compound danshen tablet on the expression of Abeta in transgenic cell model of Alzheimer's disease / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To study the effects of Compound Danshen Tablet (CDT) on the expression of the beta-amyloid peptide (Abeta) in transgenic cell model of Alzheimer's disease (AD).</p><p><b>METHODS</b>The previously successfully cultured transgenic cell model was used as AD transgenic cell model. Rats were fed with 0.5, 1, 2, and 4 times CDT. The AD transgenic cells were cultured in 0.5, 1, 2, and 4 times rats' sera. The expressions of Abeta in transgenic cells were detected using Western blot.</p><p><b>RESULTS</b>The optimal dose effect was obtained in the 1 time CDT group at 24 h.</p><p><b>CONCLUSION</b>CDT could reduce the Abeta expression level in the AD transgenic cell model.</p>

Thinking on the prevention and treatment of myocardial ischemia/reperfusion injury by Chinese medicine therapy of activating blood circulation and removing stasis / 中国中西医结合杂志

In this paper, the theoretical and experimental researches concerning the prevention and treatment of myocardial ischemia/reperfusion (I/R) injury by Chinese medicine (CM) therapy of activating blood circulation and removing stasis in recent five years were reviewed, and the mechanisms were summarized. Thereby, based upon the current development of molecular biology and application of new technology, the authors offered their suggestions on the emphasized points and methods of present CM study in this scope.

Research of Distributed Regularity of TCM Syndrome in Multiple Sclerosis / 中国中医药信息杂志

Objective To investigate the distribution regularity of TCM science basic syndrome for multilple sclerosis (MS}. Methods Through retrospective analysis for general data of case 223 patients and the information of TCM diagnosis with the method of case analysis and modem mathematical statistic, the syndrome types of the case collected were established. Results General fatigue, weariness and no desire to speak, quadriplegia, megrim, acroanesthesia and so on, which reflect the main symptoms of MS, with occurrence frequency of above 60 percent. Five basic symptoms: Syndrome of haemostasis blocking collaterals (18.0%), dark purple tongue is the most important factor; Syndrome of dampness-heat blocking collaterals (17.2%), yellow greasy furred tongue is the important factor; Syndrome of yin-asthenia and obstruction of collateral (14.8%), rapid and thining pulse is the important factor; Syndrome of endogenous wind of liver and obstruction of collateral due to wind-phlegm (14.1%), physical convulsion is the important factor; Syndrome of asthenia of both qi and blood, and channel qi unfavorable (35.9%), feeble pulse is the important factor. Conclusion The location of MS is channels and collaterals. The characteristic of MS is haemostasis, moist heat, deficiency of yin and wind-sputum, deficiency of qi and blood. General fatigue, weariness and no desire to speak, quadriplegia, megrim, acroanesthesia and so on are the main characteristics of MS.