ABSTRACT
<p><b>AIM</b>To explore a role of G6PD in replenishment of intracellular GSH during oxidative stress.</p><p><b>METHODS</b>In vitro Raji cell was cultured, intracellular GSH levels and G6PD, GR, GPX activities were determined at different time points after PMS treatment when G6PD activity was inhibited or not by DHEA.</p><p><b>RESULTS</b>Intracellular GR, GPX, G6PD activities elevated significantly combined with GSH level decreased dramatically before 30 minutes, replenished gradually after 30 minutes and restore normal levels about 6 h after PMS treatment when G6PD was not inhibited. No change in GR and significant increase in GPX activity were shown following depleted GSH after PMS treatment when G6PD was inhibited by DHEA.</p><p><b>CONCLUSION</b>G6PD contributes to replenish intracellular GSH and is a critical factor regulating GSH levels during oxidative stress.</p>