ABSTRACT
To study the immunomodulatory activity of saline extracts of leaves of Aloe vera Linn. (Family: Liliaceae) on the albino mice. The saline extract of leaves of Aloe vera was administered orally according to their body weight in mice. The assessment of immunomodulatory activity on specific and nonspecific immunity was studied by administration of test extract. The method of pyrogallol induced immunosupression was employed with slight modification to study the immunomodulatory potential of the extract. Humoral antibody response to SRBC measurement of antibody titer by haemagglutination reaction was done and cellular immune response (Foot pad reaction test) the edema was induced in the right paw of mice by injecting SRBC (0.025x109 cells) in the sub planar region. Pyrogallol-induced suppression of humoral as well as cell mediated immune response was significantly attenuated by daily oral treatment with saline extract of Aloe vera. Vitamin E treated group exhibited similar attenuation of the suppression in immune responses. Aloe vera extract at the dose of 100 mg/kg was found to suppress delayed type hypersensitivity reaction induced by SRBCs in mice. As evidenced by marked increase in haemagglutination titers in mice was also observed. The study demonstrates that A. vera triggers both specific and non-specific responses to a greater extent. The study comprised the acute toxicity and preliminary phytochemical screening of A. vera. From the results obtained and phytochemical studies the immunostimulant effect of Aloe vera could be attributed to the alkaloids content.
ABSTRACT
The present research endeavor was towards the enhancement of solubility of nifedipine by solid dispersion method, were prepared by solvent evaporation method and polymers Poloxamer 407 was tried with different proportion with drug and increasing the different sodium lauryl sulphate (SLS) percentages. There was significant increase of dissolution rate of nifedipine, in SD of nifedipine + Poloxamer 407 (1:4) than nifedipine + Poloxamer 407 (1:2) and nifedipine + Poloxamer 407 (1:3). Solid dispersion of nifedipine was evaluated by solubility test, DSC, IR and dissolution characteristics. Solid Dispersion of Nifedipine in Poloxamer 407 improved the dissolution rate of nifedipine, which helps to enhancing solubility of Nifedipine. Dissolution rate of pure nifedipine increased, with increasing the various Polymers content and with increasing the various sodium lauryl sulphates (SLS) content. The solubility of pure nifedipine was observed in pH 7.2 ± 0.2 buffer solution, with increasing the polymer ratio as 1:2, 1:3, 1:4 and with increasing the SLS percentage(%) as 1%, 3% and 5% respectively.