ABSTRACT
Objective To measure the lethal dosage values ( LD50 ) of i.v.asarone injection for mice and to establish a standard for abnormal toxicity test of asarone injection to potentially reduce the occurrence of adverse drug reaction.Methods To obtain the LD50 value, a weighted linear probit regression method ( Bliss method) is employed. The limit of abnormal toxicity test is determined according to Appendix XI C in its 2010 edition of the Chinese pharmacopoeia.Results It is found that the LD50 of intravenously asarone injection in mice ranges from 51.9 to 153.1 mg/kg.The abnormal toxicity test should be added as an additional item in the standard.Conclusions Based on analyses in this study, an appropriate limit of abnormal toxicity test is 15 mg/kg, which is also in line with current medical standard in China.
ABSTRACT
Objective To evaluate the effects of 3 molluscicides namely 5%niclosamide ethanolamine granules,4%“Luo-wei”(Tea-seed distilled saponins,TDS)and 50%wettable powder of niclosamide ethanolamine salt(WPN)by spraying and pou-drage methods in marshland and lake regions in dry season. Methods An environment with a high snail density was chosen as the experimental field,then the spraying and poudrage experiments were carried out in the field to assess the molluscicidal effects of 5%niclosamide ethanolamine granules,TDS and WPN delivered by spraying and poudrage methods with a concentration of 30, 6 and 2 g/m2,respectively,and the molluscicidal effects of the 3 drugs by the two methods were evaluated and compared in differ-ent time. Results After the delivery for 15 d,the adjusted snail death rates of the 5%niclosamide ethanolamine granules,TDS and WPN in the spraying experiment were 79.00%,82.29%and 84.83%,respectively,and those in the poudrage experiments were 97.42%,95.27% and 96.62%,respectively. Conclusion The molluscicidal effect of poudrage method is better than the spraying method,and the former is worthy of further extension and application in the marshland in dry season.