Spectroscopic synthetic optimizations monitoring of silver nanoparticles formation from Megaphrynium macrostachyum leaf extract

ABSTRACT Nanobiotechnology is one of the most promising areas in modern nanoscience and technology. Metallic nanoparticles have found uses in many applications in different fields, such as catalysis, photonics, electronics, medicine and agriculture. Synthesized nanoparticles through chemical and physical methods are expensive and have low biocompatibility. In the present study, silver nanoparticles have been synthesized from Megaphrynium macrostachyum (Benth. & Hook. f.) Milne-Redh., Marantaceae, leaf extract. Megaphrynium macrostachyum is a plant with large leaves found in the rainforest of West and Central Africa. Synthetic optimizations following factors such as incubation time, temperature, pH, extract and silver ion concentration during silver formation are discussed. UV–visible spectra gave surface plasmon resonance for synthesized silver nanoparticles based Megaphrynium macrostachyum peaks at 400–450 nm. X-ray diffraction revealed the average size of pure crystallites composed from Ag and AgCl.

Phytochemistry and in vitro Antimicrobial, Antioxydant Activities of Entandrophragma candollei H.

The research work was conducted to investigate the in vitro antimicrobial and antioxidant activity of extract of E. candollei (Family-Meliaceae). Phytochemical screening was done. Disc diffusion technique was used for in vitro antibacterial and antifungal screening. Minimal inhibitory concentration of promising extracts was determined by broth microdilution method. The phytochemical screening showed the presence of flavonoids compounds, tannins and saponins. The inhibition diameters were ranging from 7 to 17 mm and Minimum Inhibitory Concentrations (MIC) from 156.25 to 2500 μg/mL. The ethyl acetate and ethanolic extract revealed a high radical scavenging activity with IC50 ranging from 9.1026 to 11.8298 μg/mL. The Ferric reducing power activity was obtained with active concentration ranging from 12.5 to 200 μg/mL. These results suggest that extracts from E. candollei possess antibacterial and antioxidant activities and therefore justifies their usage in traditional medicine for the treatment of various diseases.