ABSTRACT
A total of 189 stool samples from swine with diarrhea, collected in various porcine farms in the central region of China were tested for porcine enteric caliciviruses (PEC) member porcine sapoviruses (SaV) by reverse transcription polymerase chain reaction (RT-PCR) amplification using primers designed to detect porcine SaV. Selected amplicons were sequenced to establish phylogenetic relationships with reference strains. Porcine SaV were detected in 12.70% (24/189) of the samples. Phylogenetic studies based on partial RNA polymerase gene sequences indicated that the field strains of viruses isolated in China were closely related (75.6 88.3% identity) to the porcine SaV Cowden reference strain. These results provide evidence that caliciviruses of the genus sapovirus circulate in piglets in China, but further studies are needed to clarify their importance as cause of diarrhea. This is the first report of PEC in China.
ABSTRACT
A nutrition survey of 170 pregnant women of the Han, Miao and Buyi nationality in the middle and late pregnancy was conducted in 1984. The survey included dietary survey, physical and obstetrical examination, biochemical analysis and measurement of weight and height of the newborns of the women studied. The results showed that their calorie intake was substantially suficient to the RDA but the protein intake was inadequate. The proportion of high quality protein was more than 30% of total protein intake for the Han, but less than 25% for the other two nationalities. The calcium and vitamin B2 intakes were much lower than RDA for all the women studied. The retinol intake was only about 73% of RDA for the Han in the late pregnancy. The incidence of nutritional anaemia was 26.92-50.00% among the 170 pregnant women. The weight-height of the newborns has no significant difference among three nationalities and all indices of physical development status were in medium rank on an average.