Cloning and expression analysis on full length CDS of zeaxanthin epoxidase gene in Pseudostellaria heterophylla / 中国中药杂志

Zeaxanthin epoxidase plays an important role in indirect pathway of plant abscisic acid biosynthesis. According to the data of Pseudostellaria heterophylla transcriptome, zeaxanthin epoxidase gene was isolated and named as PhZEP. The results of bioinformatics analysis showed that the coding sequence of PhZEP was 1 263 bp long and encoded 420 amino acids. The putative protein molecular weight was 47.34 kDa and its theoretical isoelectric point was 6.64. The characteristic structure domains were predicted, including binding site of lipoprotein and flavoprotein monooxyenase. A signal peptide was discovered at the N-terminal of amino acids. The Real-time PCR revealed that PhZEP had a higher expression level in leaves than other tissues of P.heterophylla. Highly expressed PhZEP was also observed at 10 d and 40 d tuberous root after flowering. PhZEP presented a different expression after treatment with ABA, fluridone and ABA +fluridone compared to the control. The expression of PhZEP in tuberous root after ABA treatment was close to that in control group, while PhZEP showed significant up-regulation in the fluridone treatment group. In this study, the PhZEP gene from P. heterophylla was cloned and this result has important significance for its functional identification. This research provides a basis for the further analysis on functional mechanism of ABA during development of P. heterophylla.

The pilot study of radiology nursing intervention in abdominal 3-T Magnetic Resonance examination / Estudio piloto de intervención de enfermería radiológica en examen de resonancia magnética 3-t / Estudo piloto sobre a intervenção de enfermagem na radiologia em exame de ressonância magnética abdominal 3 tesla

Abstract OBJECTIVE The aim of this study was to investigate the effect of radiology nursing intervention in abdominal examination at 3-T MRI. METHOD 60 patients with abdominal diseases were divided into two groups randomly: MR nursing intervention group and control group. All the patients underwent abdominal MR examination at 3-T. The MR nursing interventions were performed in nursing intervention group. The outcomes, including one-time success rate, the ratio of diagnosable MR images and the points of image quality, were compared between these two groups. RESULTS The one-time success rates in control group and MR nursing intervention group were 66.67% and 96.67% with significant difference ( χ2 =9.017, P<0.05). The ratios of diagnosable images in the two groups were 76.67% and 96.67% with significant difference (χ2 =5.192, P<0.05). The points of MR image quality in the two groups were 1.87±0.86 and 2.33±0.55, respectively. There was significant difference between these two groups (t=-2.508, P<0.05). CONCLUSION The effective nursing intervention can make the patients cooperation better in abdominal MR examination and improve the image quality significantly.

Resumen OBJETIVO Se objetivó investigar el efecto de la intervención de enfermería radiológica en examen abdominal de IRM 3-T. MÉTODO Sesenta pacientes con enfermedades abdominales fueron divididos aleatoriamente en dos grupos: grupo intervención de enfermería RM y grupo control. Todos fueron sometidos a examen abdominal de RM 3-T. Las intervenciones de enfermería RM se efectuaron en el grupo establecido. Los resultados, incluyendo tasa de éxito en única vez, relación de imágenes de RM diagnosticables y puntos de calidad de imagen, fueron comparadas entre ambos grupos. RESULTADOS La tasa de éxito en única vez en grupo control y grupo intervención de enfermería RM fueron de 66,67% y 96,67%, expresando diferencias significativas (χ2 =9.017, P<0.05). Los puntos de calidad de imagen de RM en ambos grupos fueron 1,87±0,86 y 2,33±0,55, respectivamente. Existió diferencia significativa entre los grupos (t=-2.508, P<0.05). CONCLUSIÓN La intervención de enfermería efectiva puede mejorar la cooperación de los pacientes en examen abdominal de RM y mejorar significativamente la calidad de imagen.

Resumo OBJETIVO O objetivo deste estudo foi investigar o resultado da intervenção de enfermagem na radiologia em exame de ressonância magnética (RM) abdominal 3 Tesla. MÉTODO 60 pacientes com patologias abdominais foram aleatoriamente divididos em dois grupos: grupo de intervenção de enfermagem em RM e grupo controle. Todos os pacientes foram submetidos ao exame de RM abdominal 3 Tesla. As intervenções de enfermagem em RM foram realizadas no grupo de intervenção de enfermagem. Os resultados foram comparados entre os dois grupos, incluindo a taxa de sucesso único, o coeficiente das imagens de RM diagnosticáveis e os pontos da qualidade de imagem. RESULTADOS As taxas de sucesso único no grupo controle e no grupo de intervenção de enfermagem em RM foram 66,67% e 96,67%, com diferença significativa de (χ2 =9,017, P<0,05). Os coeficientes das imagens diagnosticáveis nos dois grupos foram 76,67% e 96,67%, com diferença significativa de (χ2 =5,192, P<0,05). Os pontos da qualidade de imagem da RM nos dois grupos foram respectivamente 1,87±0,86 e 2,33±0,55. Houve uma diferença significativa entre estes dois grupos (t=-2,508, P<0,05). CONCLUSÃO A intervenção de enfermagem eficaz pode melhorar a cooperação dos pacientes no exame de RM abdominal e melhorar consideravelmente a qualidade da imagem.

Cloning and bioinformatics analysis of abscisic acid 8'-hydroxylase from Pseudostellariae Radix / 中国中药杂志

Abscisic acid 8'-hydroxylase was one of key enzymes genes in the metabolism of abscisic acid (ABA). Seven menbers of abscisic acid 8'-hydroxylase were identified from Pseudostellaria heterophylla transcriptome sequencing results by using sequence homology. The expression profiles of these genes were analyzed by transcriptome data. The coding sequence of ABA8ox1 was cloned and analyzed by informational technology. The full-length cDNA of ABA8ox1 was 1 401 bp,with 480 encoded amino acids. The predicated isoelectric point (pI) and relative molecular mass (MW) were 8.55 and 53 kDa,respectively. Transmembrane structure analysis showed that there were 21 amino acids in-side and 445 amino acids out-side. High level of transcripts can detect in bark of root and fibrous root. Multi-alignment and phylogenetic analysis both show that ABA8ox1 had a high similarity with the CYP707As from other plants,especially with AtCYP707A1 and AtCYP707A3 in Arabidopsis thaliana. These results lay a foundation for molecular mechanism of tuberous root expanding and response to adversity stress.

Cloning and expression analysis of four carotenoid cleavage dioxygenases genes from Pseudostellaria heterophylla / 中国中药杂志

To investigate the molecular mechanism of quality formation of Pseudostellaria heterophylla, the carotenoid cleavage dioxygenases (CCDs) genes were cloned from the transcriptome database of P. heterophylla, and analyzed them with bioinformatics analysis and expression analysis. The sequence length of four new gene were 1 617, 1 461, 1 746, 1 875 bp, and subsequently, named as PhCCD1,PhNCED2,PhNCED3 and PhCCD4 according to its genetic relationship with Arabidopsis thaliana. The sequence analysis showed that four new gene were all containing REP65 domains and binding sites of ferrous ion, such as histidine, glutamates and aspartates. Analysis phylogeny showed that PhNCED2 and PhNCED3 were the cluster of NCEDs, PhCCD1 and PhCCD4 were the cluster of CCDs. In addition, PhCCD1 and AtCDD1 of Arabidopsis thaliana, PhCCD4 and AtCCD4 of A. thaliana,PhNCED2, PhNCED3 and AtNCED3 of A. thaliana have high similarities. Analysis of real-time fluorescence quantitative showed that PhNCED2 and PhNCED3 were expressed mainly in underground part, the expression quantity of PhNCED2 reached the highest in fibrous root, PhNCED3 keeps higher in phloem and xylem, it may be the key enzymes of ABA biosynthesis genes. Moreover,PhCCD1 and PhCCD4 were expressed mainly in aerial part,the expression quantity of PhCCD1 reached the highest in leaf,PhCCD4 keeps higher in stem and leaf.It may be involved in the biosynthesis of carotenoids for P. heterophylla. The study obtained CDDs gene of P. heterophylla for the first time,this would lay the foundation of developing the response mechanism of P. heterophylla about external stress further,and then exploring the biological approach of quality formation in P. heterophylla.

Role of Diffusion Weighted Magnetic Resonance Imaging at 3-T in Staging of Endometrial Cancer and Correlation to Histopathology.

Aim: To investigate the value of diffusion weighted imaging (DWI) at 3-T MR in staging of endometrial cancer and the correlation to histopathology. Study Design: A retrospective study. Place and Duration of Study: CT-MR Division, Qianfoshan Hospital Affiliated to Shandong University. Department of Radiology, Affiliated Hospital of Jining Medical College. From June 2013 to June 2014 Methodology: 30 patients with histologically proved endometrial cancers were analyzed retrospectively. The staging diagnosis of DWI was compared with pathologic results. The ADC values in different histologic types and different differentiated of endometrial cancers were also compared. P<0.05 was considered statistically significant. Results: The staging accuracy of DWI was 83.3%. The ADC value in 30 patients of endometrial cancer was (0.856±0.080) ×10-3 mm2/s. There was no statistically significant difference in different histologic types (t=1.093，P=0.284). In different differentiated endometrial cancers, there was significant difference (F=97.246，P=0.000). Conclusion: DWI has considerable value in staging of endometrial cancer. The ADC values can demonstrate the grade malignancy of tumors initially. So diffusion weighted sequences can be included in routine MR protocols for tumor assessment.