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[Summary] During the past 100 years, especially since New China was born, China has made important substantial contributions to the progress and development of medicine in the world. Dedication, hardship, innovation, and exploration were the key elements behind these significant achievements. Medical universities should energetically train and give incentives to the talented young people and encourage their innovation, while medical students and young doctors should establish and forge ahead with determination, persist in exploring new frontiers, and make even more remarkable achievements in the future.
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Objective To identify biomarkers in cerebrospinal fluid (CSF) by proteomic technology and develop a diagnostic model for neuropsychiatric lupus (NPSLE).Methods CSF proteomic spectra of 27 patients with NPSLE before and after treatment,and 27 controls including 17 patients with scoliosis,and 10 SLE patients without neuropsychiatric manifestation (non-NPSLE) were generated by matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) combined with weak cationic exchange (WCX) magnetic beads.Data were analyzed with t test,non-parametric Kruskal-Wallis H test or Wilcoxon sign-rank test.A decision tree model for NPSLE classification was built based on the discriminating peaks.In addition,CSF samples of 12 patients with NPSLE,12 patients with lumbar disc herniation and 9 patients with other neurological conditions were employed as blind test group to verify the accuracy of the model.Results Twelve discriminating mass-to-charge (m/z) peaks were identified between NPSLE and controls.The diagnostic decision tree model,built with a panel of m/z peaks 8595,7170,7661,7740 and 5806,recognized NPSLE with the sensitivity and specificity of 92.6％ and 92.6％ based on training group samples,91.7％ and 85.7％ based on blind test group,respectively.Conclusion Potential CSF NPSLE biomarkers are identified by proteomic technology,the novel diagnostic model is sensitive and relatively specific for the diagnosis of NPSLE.
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Objective To express functional haemegglutinin(HA)protein in two different bacularvirus expression systems.Methods The whole open reading frame of A/Sichuan/1/2009(H1N1)HA was obtained by synthesis,and the HA protein were expressed in insect cells by two different bacularvius expression systems:BaculoGold system and Bac-to-Bac system. Soluble HA protein was identified by Western blot and haemegglutination test. Results The correct full length of HA gene was obtained and cloned into pAcGP67B and pFAST Bacl vectors,respectively.After 3 rounds of virus amplifyjng by re-infection of Sf9 cells,the HA protein was detected in supematant of BaculoGold system and in intracellular of Bac-to-Bac system which is much better than the former.Purified HA protein was positive not only identified by Western blot,but also detected by haemegglutinin test. Conclusion Functional HA protein was successfully expressed in two distinct bacularvirus expression systems,of which the Bac-to-Bac bacularvirus expression system is more suitable for expression of A/Sichuan/1/2009(H1N1)HA protein.
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It has been shown that rat splenocytes incubated in IL-2 beoome lytic for WBT-2M fibrosarcoma cells of WKA rats and for SST-2 mammary carcinoma cells of SHR rats. We have performed the adoptive transfer of such LAK cells in WBT-2M and SST-2 met astasis models to test their in vivo efficacy. The LAK cells infused i. v. into WKA rats with WBT-2M fibrosarcoma and into SHR rats with SST-2 mammary carcinoma, led to marked decrease in the number of pulmonary metastatic nodules and to improve the survival of animals bearing SST-2. The combination of LAK cells and IL-2 provided a more effective antimetatic activity than LAK alone. T