ABSTRACT
We report here on a case of a RhD blood group phenotype with anti-D. The RhD phenotype for partial D phenotyping with using six monoclonal anti-sera typed as normal RhD for this case. DNA sequencing analysis of the RhD gene covering intron 8 to exon 10 showed two AAATAAGATA insertion sites in intron 8 and a single nucleotide change in the exon 10 area as compared with the normal RhD gene. However, the functional role of the RhD antigen is unclear.
Subject(s)
Exons , Genotype , Introns , Isoantibodies , Phenotype , Sequence Analysis, DNAABSTRACT
BACKGROUND: Pre-transfusion tests are important for performing safe transfusion and there is a need to standardize the process of these tests. In blood banks, automation of tests is under developed and there is always a high risk of error. Automatic instruments for blood bank tests are used in blood centers where a great volume of tests are performed. However, other small scale hospitals have little experience with automatic blood bank instruments. Here, we evaluated the newly developed automatic instrument, the AutoVue Innova (Ortho-Clinical Diagnostics, Raritan, NJ, USA), for performing unexpected antibody screening tests and we compared this to other well known systems. METHODS: In a comparative study, a total of 136 samples, including 28 antibody screening positive samples and 108 negative samples, were tested in parallel by the LISS/Coombs card (DiaMed Ag, Cresssier, Morat, Switzerland) and the AutoVue Innova. The positive samples that were proven by the LISS/Coombs card and the AutoVue Innova were identified by the ID-DiaCell panel (DiaMed Ag, Cresssier, Morat, Switzerland) and the 0.8% Resolve Panel A (Ortho, Raritan, NJ, USA), respectively. Discrepant samples were rechecked by the Identisera Diana (Diagnostic Grifols, Barcelona, Spain). RESULTS: Among the 136 samples, 134 results (98.5%) of the AutoVue Innova agreed with those of the LISS/Coombs card and 2 results were discrepant. These two results were antibody screening positive only on the AutoVue and they were identified as being anti-Lewis(a). CONCLUSION: The unexpected antibody screening tests using the AutoVue Innova showed reliable results for general accuracy and they were useful in aspect of a decreased workload and increased safety, and even for less experienced persons.
Subject(s)
Automation , Blood Banks , Mass ScreeningABSTRACT
BACKGROUND: Pre-transfusion tests are important for performing safe transfusion and there is a need to standardize the process of these tests. In blood banks, automation of tests is under developed and there is always a high risk of error. Automatic instruments for blood bank tests are used in blood centers where a great volume of tests are performed. However, other small scale hospitals have little experience with automatic blood bank instruments. Here, we evaluated the newly developed automatic instrument, the AutoVue Innova (Ortho-Clinical Diagnostics, Raritan, NJ, USA), for performing unexpected antibody screening tests and we compared this to other well known systems. METHODS: In a comparative study, a total of 136 samples, including 28 antibody screening positive samples and 108 negative samples, were tested in parallel by the LISS/Coombs card (DiaMed Ag, Cresssier, Morat, Switzerland) and the AutoVue Innova. The positive samples that were proven by the LISS/Coombs card and the AutoVue Innova were identified by the ID-DiaCell panel (DiaMed Ag, Cresssier, Morat, Switzerland) and the 0.8% Resolve Panel A (Ortho, Raritan, NJ, USA), respectively. Discrepant samples were rechecked by the Identisera Diana (Diagnostic Grifols, Barcelona, Spain). RESULTS: Among the 136 samples, 134 results (98.5%) of the AutoVue Innova agreed with those of the LISS/Coombs card and 2 results were discrepant. These two results were antibody screening positive only on the AutoVue and they were identified as being anti-Lewis(a). CONCLUSION: The unexpected antibody screening tests using the AutoVue Innova showed reliable results for general accuracy and they were useful in aspect of a decreased workload and increased safety, and even for less experienced persons.
Subject(s)
Automation , Blood Banks , Mass ScreeningABSTRACT
BACKGROUND: Vacuum tubes are widely used in the clinical laboratory for routine tests. We compared a newly developed Green Vac-Tube (SPM, Gimje, Korea) with Vacutainer (BD, Franklin Lakes, NJ, USA) and Vacuette (Greiner Bio-One, Frickenhausen, Germany) in routine chemistry and hematology tests. METHODS: A total of 101 volunteers, 81 patients and 20 healthy volunteer, were recruited and we had collected blood samples with three kinds of EDTA tubes and those of serum separating tubes. The samples were evaluated for chemistry and hematology tests using TOSHIBA 200FR (Toshiba, Tokyo, Japan) and ADVIA (Siemens, Deerfield, IL, USA) respectively. Their results were statistically analyzed by paired t-test and Bland-Altman plot. RESULTS: Their clinical utilities were examined by CLIA'88 programs. Paired t-test analysis revealed that the results of ALP, AST, total bilirubin, CO2, Hct and MCV showed statistically significant differences between Green Vac-Tube and previously used two vacuum tubes. Similar significant differences were also observed between previous two vacuum tubes. And 194 (4.37%) cases among 5,151 cases were in the critical region by Bland-Altman plot. All different cases, except Na+, K+ however, were clinically acceptable by CLIA'88 programs. CONCLUSIONS: Green Vac-tube has good analytical performance compared to previously-used tubes.