ABSTRACT
BACKGROUND:Morinda has been reported to promote the proliferation, the secretion of alkaline phosphatase and osteocalcin, and mRNA expression of transforming growth factor of osteoblasts. However, little information is available addressing the effects of Morinda on receptor activator of nuclear factor-κB expression in osteoclasts in rats with osteoporosis. OBJECTIVE:To study the effects of Morinda on receptor activator of nuclear factor-κB expression in osteoclastsofosteoporosis rats. METHODS:Thirty Sprague-Dawley rats were equaly and randomly divided into Morinda and 17β-estradiol groups. Rat models of osteoporosis were established by bilateral ovariectomy, and then 5 mL of Morinda decocta(1.0mmol/L)and 17β-estradiol (1×10-6mmol/L) were administered intragastricaly to rats in Morinda and 17β-estradiol groups for 3 consecutive months, respectively. Primary osteoclasts were isolated from rats in both groups, andthen cultured for 3, 6 and 9 days folowed by TRAP staining andcelcounting. Bone mineral density of the proximal and distal femur, urine and serum levels of Ca2+and progesterone, and receptor activator of nuclear factor-κB expression in osteoclasts ofrats in both groups were determined. RESULTS AND CONCLUSION:Osteoclast fusion was reduced in Morinda group. In contrast, number of osteoclastswas increased andcels becamemore maturein the17β-estradiol group. Bone mineral density of the proximal and distal femur bilateraly, urine and serum levels of Ca2+and progesterone were significantly increased, while receptor activator of nuclear factor-κB expression was significantly decreased in osteoclasts in Morinda group compared with 17β-estradiol group (P< 0.05). These results indicate that Morinda reduces receptor activator of nuclear factor-κB expression in osteoclasts in osteoporosis rats, thereby inhibiting the development and progression of osteoporosis.
ABSTRACT
BACKGROUND:There is no consensus on the choice of elastic stable intramedulary nailing or plate fixation for the treatment of humeral fractures in children.Current research is limited to smal-sample studies,and it is difficult to carry out a large-sample multicenter analysis.OBJECTIVE:To evaluate the efficacy and safety of elastic stable intramedulary nailing and plate fixation for the treatment of humeral fractures in children with meta-analysis.METHODS: The PubMed database,EMbase database,CBM database,CNKI database,VIP database and Wangfang database were searched with computer to colect the controled trials of elastic stable intramedulary nailingversusplate fixation for humerus fractures in children,and related journals were manualy searched.The searching time ranged from the date of database establishment to August 2014.The trails were selected,the data were extracted and the quality was evaluated by two investigators independently.RESULTS AND CONCLUSION: Two randomized controled trials and three retrospective controled trials were included in the meta-analysis.The Meta-analysis results showed that the postoperative functional recovery Constant score of the elastic stable intramedulary nailing group was higher than that of the plate fixation group (P＜0.01).The bone union time,operation time,incision length,intraoperative blood loss and the hospital stay of theelastic stable intramedulary nailing group were less than those of the plate fixation group (P＜0.01).There were no significant differences in incidence rate of complications,nonunion,wound infection and malunion between two groups (P＞0.05).Based on the current evidence,elastic stable intramedulary nailing for the treatment of humeral fractures in children is superior to the plate fixation in the efficacy.There is no significant difference in incidence rate of complications between elastic stable intramedulary nailing and plate fixation.But al the studies were smal-sample,and high-risk original study.Clinical trials with adequate samples,rational design and strict execution shal be carried out to provide more reliable evidence.