ABSTRACT
Many beneficial medicinal products are used to treat various serious diseases and disorders like diabetes, cancer and cardiovascular diseases without side effects. Hence our study focused to investigate the phytochemical analysis, quantification of bioactive compounds (alkaloid, phenols, flavonoids), in vitro free radical scavenging activity, anti-inflammatory activity (Membrane stabilization assay), in vitro anti-diabetic activity by enzyme inhibition activity, glucose uptake assay in isolated rat hemi-diaphragm and in yeast cells and also record GC-MS profile of Erythrina variegata L. bark (methanolic extract) which has boundless medicinal properties. The results of this study showed the evidence that the extracts when tested for their phytochemicals and free radical scavenging activity were found to have considerable antioxidant potential. This plant also exhibit better in vitro enzyme inhibitory activity, inhibition of non- enzymatic glycosylation of hemoglobin, in vitro anti-diabetic activity proved by glucose uptake assays and anti-inflammatory activity. GC-MS analysis of methanolic extract of Erythrina variegata L. bark revealed the presence of 58 compounds which are responsible for pharmacological activities. The results of this study indicate that the methanolic extract of Erythrina variegata L. bark has significant pharmacological properties.
ABSTRACT
The aim of the study was to investigate the invivo antioxidant activity of ethanolic extract Premna corymbosa (Rottl.) root against streptozotocin induced oxidative stress in different organs (liver, kidney, brain, heart and pancreas) of rats. Ethanolic extract of P.corymbosa (Rottl.) root was administered orally (200 mg/kg body weight) and the effect of extract on enzymatic antioxidants like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and polyphenol oxidase (PPO), non enzymatic like vitamin C, vitamin E and glutathione. Lipid peroxidation like basal, ascorbate and peroxide induced lipid peroxidation were also estimated. Glibenclamide was used as standard reference drug. A significant increase in the activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione-Stransferase and reduced glutathione were observed in different organs of diabetic rats on treatment with 200 mg/kg body weight of P.corymbosa (Rottl.) root extract and glibenclamide for 30 days treatment. Both the treated groups showed significant decrease in lipid peroxidation, suggesting its role in protection against lipid peroxidation induced membrane damage. P.corymbosa (Rottl.) possesses antioxidant potential which may be used for therapeutic purposes mainly in the prevention of oxidative damage that occur during diabetes.